Transcriptome Analysis Identifies SenZfp536,a Sense LncRNA that Suppresses Self-renewal of Cortical Neural Progenitors

作     者：Kuan Tian Andi Wang Junbao Wang Wei Li Wenchen Shen Yamu Li Zhiyuan Luo Ying Liu Yan Zhou Kuan Tian;Andi Wang;Junbao Wang;Wei Li;Wenchen Shen;Yamu Li;Zhiyuan Luo;Ying Liu;Yan Zhou

作者机构：College of Life SciencesRenmin Hospital of Wuhan UniversityWuhan UniversityWuhan430072China Frontier Science Center for Immunology and MetabolismMedical Research InstituteSchool of MedicineWuhan UniversityWuhan430071China Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2021年第37卷第2期

页      面：183-200页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

基　　金：This work was supported by grants from the National Key R&D Program of China(2018YFA0800700) the National Natural Science Foundation of China(31970770,31970676,and 31671418) the Natural Science Foundation of Hubei Province,China(2018CFA016) Fundamental Research Funds for the Central Universities,the Medical Science Advancement Program(Basic Medical Sciences)of Wuhan University(TFJC2018005) State Key Laboratory Special Fund 2060204 

主　　题：Zfp536 Sense lncRNA Self-renewal Cortical development Neural progenitor 

摘      要：Long non-coding RNAs(lncRNAs)regulate transcription to control development and homeostasis in a variety of tissues and ***,their roles in the development of the cerebral cortex have not been well ***,a bioinformatics pipeline was applied to delineate the dynamic expression and potential cis-regulating effects of mouse lncRNAs using transcriptome data from 8 embryonic time points and sub-regions of the developing cerebral *** further characterized a sense lncRNA,SenZfp536,which is transcribed downstream of and partially overlaps with the protein-coding gene *** SenZfp536 and Zfp536 were predominantly expressed in the proliferative zone of the developing ***536 was cis-regulated by SenZfp536,which facilitates looping between the promoter of Zfp536 and the genomic region that transcribes ***,knocking down or activating the expression of SenZfp536 increased or compromised the proliferation of cortical neural progenitor cells(NPCs),***,overexpressing Zfp536 in cortical NPCs reversed the enhanced proliferation of cortical NPCs caused by SenZfp536 *** study deepens our understanding of how lncRNAs regulate the propagation of cortical NPCs through cis-regulatory mechanisms.