Molecular Analysis of Pathogenicity Differences of Avian Paramyxovirus 1 Genotypes VI and VII in Chickens

作     者：Yang Song Jing Zhao Huiming Yang Yawen Bu Guozhong Zhang 

作者机构：Key Laboratory of Animal Epidemiology of the Ministry of AgricultureCollege of Veterinary MedicineChina Agricultural UniversityBeijing100193China 

出 版 物：《Veterinary Science Research》 (兽医科学研究（英文）)

年 卷 期：2019年第1卷第1期

页      面：1-7页

学科分类：0906[农学-兽医学] 09[农学] 

基　　金：This study was supported by the Beijing Agriculture Innovation Consortium of Poultry Research System(BAIC04-2019) We thank Ye Zhao,PhD,from China Agricultural University for editing a draft of this manuscript 

主　　题：Avian avulavirus-1 Genotype Pathogenicity Chicken Molecular determinant 

摘      要：Background:Genotypes VI and VII of(APMV-1)have different host range and pathogenicity in pigeons and ***,the molecular determinants of these differences are still ***:Here,we aligned the DNA sequences of 56 genotype VI and 33 genotype VII APMV-1 *** alignment results revealed that there are 17 amino acids sites differed between APMV-1 strains of these two *** then constructed a plasmid based on the full-length genome of rSG10 APMV-1 strain,which belongs to genotype VII but was mutated with these 17 VI-genotype-specific amino acids,and rescued as rSG10-17 *** restriction digestion and ligation and overlapping PCR methods were used in the construction of plasmids with amino acids *** virus was evaluated for its virulence and growth *** and conclusion:The results indicated that the virulence and the growth characteristics have no obvious difference between the rSG10-17 virus and its parental strain *** simultaneous mutation of 17 genotype-specific amino acids did not affect the virulence of APMV-1 in *** analysis of these amino acids is required by taking into consideration of the functions of encoded proteins.