Ginsenoside Rb1 Pretreatment Attenuates Myocardial Ischemia by Reducing Calcium/Calmodulin-Dependent Protein Kinase Ⅱ-Medicated Calcium Release

作     者：Wen-Jun Zhou Juan-Li Li Qian-Mei Zhou Fei-Fei Cai Xiao-Le Chen Yi-Yu Lu Ming Zhao Shi-Bing Su Wen?Jun Zhou;Juan?Li Li;Qian?Mei Zhou;Fei?Fei Cai;Xiao?Le Chen;Yi?Yu Lu;Ming Zhao;Shi?Bing Su

作者机构：Research Center for Traditional Chinese Medicine Complexity SystemInstitute of Interdisciplinary Integrative Medicine ResearchShanghai University of Traditional Chinese MedicineShanghaiChina AntiCancerInc.San DiegoUSA 

出 版 物：《World Journal of Traditional Chinese Medicine》 (世界中医药杂志（英文）)

年 卷 期：2020年第6卷第3期

页      面：284-294页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Natural Science Funds(81073134) 085 First-Class Discipline Construction Innovation Science and Technology Support Project of Shanghai University of TCM(085ZY1206) E-institutes of Shanghai Municipal Education Commission(No E 03008) 

主　　题：Calcium/calmodulin-dependent protein kinaseⅡ Ginsenoside Rb1 myocardial ischemia ryanodine receptor 

摘      要：Objective:The aim of this study was to investigate the protective effects of ginsenoside Rb1 and assess whether these protective effects are related to calcium/calmodulin-dependent protein kinaseⅡ(Ca MKⅡ).Methods:A myocardial ischemia(IS)*** and a myocardial H9 C2 cell hypoxia model were *** was induced by occluding the left anterior descending artery for 120 *** Rb1(10 mg/kg)was administered 30 min before ischemia induction,and the treatment continued for 7 ***:In the rat IS injury model,ginsenoside Rb1 reduced myocardial infarct size,mean left ventricular diastolic pressure,incidence of arrhythmia,and levels of serum creatine kinase,lactate dehydrogenase,and ***,the mean left ventricular systolic pressure,and maximal rising and falling rates of ventricular pressure(±dp/dtmax)*** the myocardial H9 C2 cell hypoxia model,ginsenoside Rb1 reduced intracellular calcium concentrations([Ca2+]i)during hypoxia,and markedly reversed the hypoxia-induced decrease in cell *** Rb1 was involved in the downregulation of CaMKⅡand the ryanodine receptor,as well as hypoxia-induced H9 C2 cell ***:The findings of the present study suggest that ginsenoside Rb1 attenuates MI injury in rats,partially through the downregulation of CaMKⅡexpression.