A Retrospective Analysis for Different Routes of Administration in Mice-Percutaneous Retro-Orbital, Jugular Catheter, Tail Vein and Femoral Cut Down Injections

作     者：Priyanka Prathipati Cristian Rodriguez-Aguayo Brian L Walton Anil K. Sood Jamieson Greaver Christopher F. Janssen Gabriel Lopez-Berestein Priyanka Prathipati;Cristian Rodriguez-Aguayo;Brian L Walton;Anil K. Sood;Jamieson Greaver;Christopher F. Janssen;Gabriel Lopez-Berestein

作者机构：Department of Advanced Cardiopulmonary Therapies and Transplantation Center for Advanced Heart Failure University of Texas Health Science Center at Houston Houston TX USA Department of Experimental Therapeutics University of Texas M.D. Anderson Cancer Center Houston TX USA The Center for RNA Interference and Non-Coding RNA University of Texas M.D. Anderson Cancer Center Houston TX USA Department of Cancer Biology University of Texas M.D. Anderson Cancer Center Houston TX USA Department of Gynecology Oncology & Reproductive Medicine University of Texas M.D. Anderson Cancer Center Houston TX USA Interdisciplinary Research Center for Laboratory Animal Medicine and Care University of Texas Health Science Center at Houston Houston TX USA 

出 版 物：《Journal of Biosciences and Medicines》 (生物科学与医学（英文）)

年 卷 期：2020年第8卷第9期

页      面：131-141页

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：Femoral Vein Jugular Catheter Liposomes Retro-Orbital Tail Vein 

摘      要：Liposomes effectively transport fatty proteins to targeted tissues. Laboratory experiments use multiple methods to administer liposomes, but comparison of these methods is not available. In this retrospective study, we characterized and compared four intravenous administration routes (tail vein, jugular catheter, femoral vein and percutaneous retro-orbital injections) in murine models. ApoE-/- mice were used to compare administration routes. Results indicate that the jugular catheter route delivered the highest amount of liposomes to tissues due to longer period of injections compared to other routes;however, this route failed to remain patent for 8/10 animals. Delivery via tail vein, femoral vein and percutaneous retro-orbital injections resulted in similar accumulation in the organs. When including technical difficulty and expense, percutaneous retro-orbital injections of liposomes are the most convenient and efficacious approach.