Screening and Characterization of Antibiotic Producing Organisms from Waste Dump Soil Sample
Screening and Characterization of Antibiotic Producing Organisms from Waste Dump Soil Sample作者机构：Department of Applied Microbiology and Brewing Nnamdi Azikiwe University Awka Nigeria Department of Biological Sciences Godfrey Okoye University Enugu Enugu State Nigeria Department of Microbiology Novena University Ogume Delta State Nigeria Department of Microbiology Renaissance University Ugbawka Enugu State Nigeria Department of Pharmaceutical Microbiology and Biotechnology Faculty of Pharmaceutical Sciences Chukwuemeka Odumegwu Ojukwu University Igbariam Anambra State Nigeria
出 版 物：《Advances in Microbiology》 (微生物学（英文）)
年 卷 期：2020年第10卷第9期
页 面：422-433页
学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学]
主 题：Antibiotics Crude Extract Ciprofloxacin Soil Sample
摘 要：This study was designed to screen and characterize antibiotic producing bacteria and fungi from soil samples from a waste dump site in Enugu. The molecular characterization of the isolates was performed by using Zymo Research Quick-DNA fungi/bacteria miniprep kit. The bacteria isolates were Providencia stuartii, Providencia alcalifaciens. Bacillus sp., Streptococcus sp., Micrococcus sp., and Staphylococcus sp while Aspergillus fumigatus and Aspergillus niger were fungal isolates. The efficacy of the Crude Extract (CE) of the antibiotics produced was tested against species of Staphylococcus aureus and Escherichia coli. The CE of antibiotics when compared with a conventional antibiotic (Ciprofloxacin) showed lower antimicrobial activity. The minimum inhibitory concentration of the crude extracts ranged between 12.5% and 25% concentrations while the minimum bactericidal concentration ranged from 25% and 50% concentrations. The findings suggest that these organisms have the potential to produce antibiotics and could be harnessed by pharmaceutical industries for the production of newer antibiotics.
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