Long-Term Existence of SARS-CoV-2 in COVID-19 Patients:Host Immunity,Viral Virulence,and Transmissibility

作     者：Xingyu Wang Kun Huang Haini Jiang Lijuan Hua Weiwei Yu Dan Ding Ke Wang Xiaopan Li Zhong Zou Meilin Jin Shuyun Xu Xingyu Wang;Kun Huang;Haini Jiang;Lijuan Hua;Weiwei Yu;Dan Ding;Ke Wang;Xiaopan Li;Zhong Zou;Meilin Jin;Shuyun Xu

作者机构：Division of Cardiothoracic and Vascular SurgeryTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China State Key Laboratory of Agricultural MicrobiologyHuazhong Agricultural UniversityWuhan 430070China College of Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China Medical Affairs OfficeTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Respiratory and Critical Care MedicineKey Laboratory of Pulmonary Diseases of Health MinistryTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China General DepartmentTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2020年第35卷第6期

页      面：793-802页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：support by National Natural Science Foundation of China under Grant(81800095) Key Scientific and Technological Project of Hubei province(2020FCA004) 

主　　题：SARS-CoV-2 Long-term Neutralization antibody Lymphocyte functionality Viral pathogenicity 

摘      要：COVID-19 patients can recover with a median SARS-CoV-2 clearance of 20 days post initial symptoms(PIS).However,we observed some COVID-19 patients with existing SARS-CoV-2 for more than 50 days *** study aimed to investigate the cause of viral clearance delay and the infectivity in these *** data and clinical characteristics of 22 long-term COVID-19 patients were *** median age of the studied cohort was59.83±12.94 *** patients were clinically cured after long-term SARS-CoV-2 infection ranging from 53 to112 days *** lymphocytes counts were *** ratios of interferon gamma(IFN-c)-secreting cells to total CD4^(+)and CD8^(+)cells were normal as 24.68%±9.60%and 66.41%±14.87%***,the number of IFNc-secreting NK cells diminished(58.03%±11.78%).All patients presented detectable IgG,which positively correlated with mild neutralizing activity(Mean value neutralisation antibodies titers=157.2,P=0.05).No SARS-CoV-2 virus was isolated in Vero E6 cells inoculated with nasopharyngeal swab samples from all patients 50 days PIS,and the cytopathic effect was *** one sample was positive for SARS-CoV-2 nucleic acid test in cell supernatants after two *** sequencing revealed that only three synonymous variants were identified in spike protein coding *** conclusion,decreased IFN-c production by NK cells and low neutralizing antibodies might favor SARS-CoV-2 long-term ***,low viral load and weak viral pathogenicity were observed in COVID-19 patients with long-term SARSCoV-2 infection.