Biomimetic glycosaminoglycan-based scaffolds improve skeletal muscle regeneration in a Murine volumetric muscle loss model

作     者：Naagarajan Narayanan Zhihao Jia Kun Ho Kim Liangju Kuang Paul Lengemann Gabrielle Shafer Victor Bernal-Crespo Shihuan Kuang Meng Deng 

作者机构：Department of Agricultural and Biological EngineeringPurdue UniversityWest LafayetteIN47906United States Bindley Bioscience CenterPurdue UniversityWest LafayetteIN47906United States Department of Animal SciencePurdue UniversityWest LafayetteIN47906United States Center for Comparative Translational ResearchPurdue UniversityWest LafayetteIN47906United States School of Materials EngineeringPurdue UniversityWest LafayetteIN47906United States Weldon School of Biomedical EngineeringPurdue UniversityWest LafayetteIN47906United States 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2021年第6卷第4期

页      面：1201-1213页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：NIH R03AR068108 NIH R01AR071649 and Purdue Start-up Package is greatly appreciated.The authors acknowledge the use of Purdue Life Science Microscopy Facility Purdue Histology Core Facility.The authors also acknowledge the use of facilities of the Bindley Bioscience Center a core facility of the NIH-funded Indiana Clinical and Translational Sciences Institute 

主　　题：Hyaluronic acid Chondroitin sulfate Hydrogels Volumetric muscle loss Myoblasts Skeletal muscle tissue engineering 

摘      要：Volumetric muscle loss(VML)injuries characterized by critical loss of skeletal muscle tissues result in severe functional *** treatments involving use of muscle grafts are limited by tissue availability and donor site *** this study,we designed and synthesized an implantable glycosaminoglycan-based hydrogel system consisting of thiolated hyaluronic acid(HA)and thiolated chondroitin sulfate(CS)cross-linked with poly(ethylene glycol)diacrylate to promote skeletal muscle regeneration of VML injuries in *** HA-CS hydrogels were optimized with suitable biophysical properties by fine-tuning degree of thiol group substitution to support C2C12 myoblast proliferation,myogenic differentiation and expression of myogenic markers MyoD,MyoG and ***,in vivo studies using a murine quadriceps VML model demonstrated that the HA-CS hydrogels supported integration of implants with the surrounding host tissue and facilitated migration of Pax7+satellite cells,de novo myofiber formation,angiogenesis,and innervation with minimized scar tissue formation during 4-week *** hydrogel-treated and autograft-treated mice showed similar functional improvements in treadmill performance as early as 1-week post-implantation compared to the untreated *** together,our results demonstrate the promise of HA-CS hydrogels as regenerative engineering matrices to accelerate healing of skeletal muscle injuries.