Collagen scaffolds functionalized with triple-helical peptides support 3D HUVEC culture

作     者：Jean-Daniel Malcor Emma J.Hunter Natalia Davidenko Daniel V.Bax Ruth Cameron Serena Best Sanjay Sinha Richard W.Farndale 

作者机构：Department of BiochemistryUniversity of CambridgeCambridge CB21QWUK Department of Materials Science and MetallurgyUniversity of CambridgeCambridge CB30FSUK Division of Medicine and Wellcome TrustMedical Research Council Cambridge Stem Cell InstituteUniversity of CambridgeCambridge CB20AWUK Present address:CambCol Laboratories LtdOak Lane Business ParkLittleportElyCambridgeshireCB79RPUK 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2020年第7卷第5期

页      面：471-482页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 

基　　金：supported in the Department of Biochemistry by grants from British Heart Foundation(SP/15/7/31561,FS/15/20/31335 , RG/15/4/31268) funded by EPSRC fellowship(EP/N019938/1) supported by BHF grants FS/18/46/33663 , RM/17/2/33380 funding from the Wellcome Trust and MRC to the Wellcome-Medical Research Council Cambridge Stem Cell Institute Confocal imaging of scaffolds was performed at the Microscopy Core facility at the Sainsbury Laboratory,Cambridge,UK and was supported by the Gatsby Charitable Foundation 

主　　题：collagen biomaterial endothelial cells 3D cell scaffold collagen-mimetic peptides 

摘      要：Porous biomaterials which provide a structural and biological support for cells have immense potential in tissue engineering and cell-based therapies for tissue *** biomaterials that can host endothelial cells represent promising tools for the vascularization of engineered ***-dimensional collagen scaffolds possessing controlled architecture and mechanical stiffness are obtained through freeze–drying of collagen suspensions,followed by chemical cross-linking which maintains their ***,cross-linking scaffolds renders their biological activity suboptimal for many cell types,including human umbilical vein endothelial cells(HUVECs),by inhibiting cell–collagen ***,we have improved crucial HUVEC interactions with such cross-linked collagen biomaterials by covalently coupling combinations of triple-helical peptides(THPs).These are ligands for collagen-binding cell-surface receptors(integrins or discoidin domain receptors)or secreted proteins(SPARC and von Willebrand factor).THPs enhanced HUVEC adhesion,spreading and proliferation on 2D collagen *** grafted to 3D-cross-linked collagen scaffolds promoted cell survival over seven *** study demonstrates that THP-functionalized collagen scaffolds are promising candidates for hosting endothelial cells with potential for the production of vascularized engineered tissues in regenerative medicine applications.