Laboratory Diagnosis and Monitoring the Viral Shedding of SARS-CoV-2 Infection

作     者：Yang Yang Minghui Yang Jing Yuan Fuxiang Wang Zhaoqin Wang Jinxiu Li Mingxia Zhang Jinli Wei Ling Peng Gary Wong Haixia Zheng Weibo Wu Chenguang Shen Mingfeng Liao Kai Feng Jianming Li Qianting Yang Juanjuan Zhao Lei Liu Yingxia Liu 

作者机构：Shenzhen Key Laboratory of Pathogen and ImmunityNational Clinical Research Center for Infectious DiseaseState Key Discipline of Infectious DiseaseShenzhen Third People’s HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyNo.29Bulan RoadLonggang DistrictShenzhen 518112China Institut Pasteur of ShanghaiChinese Academy of SciencesShanghai 200031China 

出 版 物：《The Innovation》 (创新（英文）)

年 卷 期：2020年第1卷第3期

页      面：56-62页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the Ministry of Science and Technology(2020YFC0846300) National Science and Technology Major Project(2017ZX10103011,2018ZX10711001) Shenzhen Science and Technology Research and Development Project(202002073000001) China Postdoctoral Science Foundation(2019T120147,2019M660836) 

主　　题：SARS-COV-2 COVID-19 MOLECULAR DIAGNOSIS RESPIRATORY SPECIMENS VIRAL SHEDDING 

摘      要：The worldwide epidemic of coronavirus disease 2019(COVID-19)is *** and accurate detection of the causative virus SARSCoV-2 is vital for the treatment and control of *** this study,the comparative sensitivity of different respiratory specimen types were retrospectively analyzed using 3,552 clinical samples from 410 COVID-19 patients confirmed by Guangdong CDC(Center for Disease Control and Prevention).Except for bronchoalveolar lavage fluid(BALF),the sputum possessed the highest positive rate(73.4%–87.5%),followed by nasal swabs(53.1%–85.3%)for both severe and mild cases during the first 14 days after illness onset(d.a.o.).Viral RNA could be detected in all BALF samples collected from the severe group within 14 *** lasted up to 46 ***,although viral RNA was negative in the upper respiratory samples,it was also positive in BALF samples in most cases from the severe group during ***,no viral RNA was detected in BALF samples from the mild *** typical ground-glass opacity observed via computed tomographic scans,no viral RNA was detected in the first three or all upper respiratory tract specimens from some COVID-19 *** conclusion,sputum is most sensitive for routine laboratory diagnosis of COVID-19,followed by nasal *** of viral RNA in BALF improves diagnostic accuracy in severe COVID-19 patients.