Mining Myb transcription factors related to wood development in Larix olgensis

作     者：Jiali Zhao Huanhuan Xiong Junhui Wang Hanguo Zhang Lei Zhang Jiali Zhao;Huanhuan Xiong;Junhui Wang;Hanguo Zhang;Lei Zhang

作者机构：State Key Laboratory of Tree Genetics and BreedingNortheast Forestry UniversityHarbin 150040People’s Republic of China State Key Laboratory of Tree Genetics and BreedingChinese Academy of ForestryBeijing 100081People’s Republic of China 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2020年第31卷第6期

页      面：2453-2461页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0907[农学-林学] 08[工学] 0829[工学-林业工程] 0901[农学-作物学] 

基　　金：国家自然科学基金 the Fundamental Research Funds for the Central Universities 国家863计划 the Fundamental Research Funds for the Central Universities 

主　　题：Larix olgensis RNA-seq Myb transcription factors Diff erentially expressed genes 

摘      要：Larix olgensis A.Henry is a fast-growing tree used for aff orestation in northeastern China and has great ecological and economic value.For studying developmental genes in the xylem of this species,we investigated the Myb transcription factor family,one of the largest families of transcription factors in plants,which plays an important role in the regulation of lignifi cation in plant secondary walls.By sequencing a L.olgensis cDNA library using the Illumina HiSeq2500 high-throughput sequencing platform,we obtained 58,683 unigene sequences,of which 16,554 unigenes were longer than 1000 bp,accounting for 28.2%of the total database.The alignment of these genes with the GO,COG,KEGG,Swiss-Prot and NR databases resulted in annotated 29,350 unigenes.We obtained a total of 1460 differentially expressed genes,of which 453 were upregulated and 1007 were downregulated at the two developmental stages analyzed.The gene annotations showed a wide range of biological functions and metabolic pathways.The 10 Myb transcription factors that were obtained from the diff erentially expressed genes were analyzed by real-time quantitative PCR(qRT-PCR).The results showed that four Myb transcription factors may be associated with xylem development in L.olgensis.Due to the large genome size of conifers,genomics research on these species has lagged behind that for other plant groups.Our data provide the basis for further studies on xylem development in L.olgensis.