Differentiation of Human Adipose-Derived Stem Cells into Endometrial Epithelial Cells

作     者：Fang Yang Wan-Lin Zhang Shu-Qiang Chen Hui-Jun Sun Jie Lu Xi-Feng Xiao Xiao-Hong Wang Yang Fang;Zhang Wan-Lin;Chen Shu-Qiang;Sun Hui-Jun;Lu Jie;Xiao Xi-Feng;Wang Xiao-Hong

作者机构：Department of Obstetrics and GynecologyReproductive Medical CenterTangdu HospitalThe Fourth Military Medical UniversityXi’an 710038China 

出 版 物：《Reproductive and Developmental Medicine》 (生殖与发育医学（英文版）)

年 卷 期：2020年第4卷第3期

页      面：137-145页

核心收录：

学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学] 

基　　金：funded by grants from the National Natural Science Foundation of China(No.81671463) the Key Research and Development Plan of Shaanxi Province(No.2017ZDCXL-SF-02-03) 

主　　题：Adipose-derived Stem Cell Endometrial Epithelial Cell Estrogen Receptorα Estrogen Receptorβ H19 β-catenin 

摘      要：Objective:This study aimed to investigate the differentiation of human adipose-derived stem cells(hASCs)into endometrial epithelial cells(EECs)under certain induction conditions and to a further step provide a promising approach for ASCs in clinical practice to the treatment of severe intrauterine ***:Four groups of hASCs were separately cultured as follows:in Group 1,hASCs were cultured in a control medium(5%fetal bovine serum[FBS]+α-minimum Eagle’s medium[α-MEM]);in Group 2,hASCs were cultured in an induction medium(5%FBS+α-MEM+[1×10-7 mol/L 17β-estradiol]+10 ng/mL transforming growth factorβ1[TGF-β1]+10 ng/mL epidermal growth factor[EGF]+10 ng/mL platelet-derived growth factor BB[PDGF-BB]);in Group 3,hASCs and human endometrium cells(hEMCs)were cocultured in the control medium;and in Group 4,hASCs and hEMCs were cocultured in the induction ***:When cocultured with hEMCs,the morphology of hASCs became similar with EECs,and the addition of factors such as EGF,TGFβ,PDGF-BB,and 17β-estradiol promoted *** study,for the first time,demonstrated estrogen receptor(ER)αand ERβexpression in hASCs and preliminarily explored changes in ERα,ERβ,β-catenin,and H19 mRNA expression during hASC ***,we concluded that H19 mRNA expression was negatively correlated with differentiation,which is seemingly related to the estrogen signaling ***:hASCs revealed the potential for differentiating to EECs when cocultured with hEMCs.