Modeling protein-protein interactions in axon initial segment to understand their potential impact on action potential initiation

作     者：Piyush Bhardwaj Don Kulasiri Sandhya Samarasinghe Piyush Bhardwaj;Don Kulasiri;Sandhya Samarasinghe

作者机构：Centre of Advanced Computational Solutions(C-fACS)Lincoln UniversityChristchurchNew Zealand Department of Molecular BiosciencesLincoln UniversityChristchurchNew Zealand 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2021年第16卷第4期

页      面：700-706页

核心收录：

学科分类：0710[理学-生物学] 100208[医学-临床检验诊断学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

主　　题：Alzheimer’s disease ankyrin-G axon initial segment casein kinase-2 microtubules voltage-gated potassium channel voltage-gated sodium channel 

摘      要：The axon initial segment(AIS)region is crucial for action potential initiation due to the presence of high-density AIS protein voltage-gated sodium channels(Nav).Nav channels comprise several serine residues responsible for the recruitment of Nav channels into the structure of AIS through interactions with ankyrin-G(AnkG).In this study,a series of computational experiments are performed to understand the role of AIS proteins casein kinase 2 and AnkG on Nav channel recruitment into the *** computational simulation results using Virtual cell software indicate that Nav channels with all serine sites available for phosphorylation bind to AnkG with strong *** the low initial concentration of AnkG and casein kinase 2,the concentration of Nav channels reduces significantly,suggesting the importance of casein kinase 2 and AnkG in the recruitment of Nav channels.