A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin

作     者：Shanshan Hou Tingting Feng Na Zhao Jiaxin Zhang Huibin Wang Ning Liang Longshan Zhao Shanshan Hou;Tingting Feng;Na Zhao;Jiaxin Zhang;Huibin Wang;Ning Liang;Longshan Zhao

作者机构：School of PharmacyShenyang Pharmaceutical UniversityShenyangLiaoning Province110016China School of Pharmaceutical EngineeringShenyang Pharmaceutical UniversityShenyangLiaoning Province110016China Institute of Pharmaceutical and Food EngineeringShanxi University of Chinese MedicineJinzhongShanxi Province030619China 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2020年第10卷第5期

页      面：482-489页

核心收录：

学科分类：1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

主　　题：Carbon nanoparticles Fluorescence quenching F€orster resonance energy transfer(FRET) Fluorescein-labelled peptide Trypsin assay 

摘      要：Herein,we report a novel sensor to detect trypsin using a purpose-designed fluorescein-labelled peptide with negatively charged carbon nanoparticles(CNPs)modified by acid *** fluorescence of the fluorescein-labelled peptide was quenched by *** sensor reacted with trypsin to cleave the peptide,resulting in the release of the dye moiety and a substantial increase in fluorescence intensity,which was dose-and time-dependent,and trypsin could be quantified ***,the biosensor has led to the development of a convenient and efficient fluorescent method to measure trypsin activity,with a detection limit of 0.7 mg/*** method allows rapid determination of trypsin activity in the normal and acute pancreatitis range,suitable for point-of-care ***,the applicability of the method has been demonstrated by detecting trypsin in spiked urine samples.