Nanovolcano microelectrode arrays: toward long- term on-demand registration of transmembrane action potentials by controlled electroporation

作     者：Benoit X.E.Desbiolles Etienne de Coulon Nicolas Maino Arnaud Bertsch Stephan Rohr Philippe Renaud Beno(ī)t X.E.Desbiolles;Etienne de Coulon;Nicolas Ma(i)no;Arnaud Bertsch;Stephan Rohr;Philippe Renaud

作者机构：Laboratory of Microsystems LMIS4Ecole Polytechnique Federale de LausanneLausanneSwitzerland Department of PhysiologyLaboratory of Cellular Optics IIUniversity of BernBernSwitzerland 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2020年第6卷第1期

页      面：578-589页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 0836[工学-生物工程] 10[医学] 

基　　金：This work was partially funded by the Swiss National Science Foundation(grant number 200021_175943 to P.R.and 310030_169234 to S.R.) 

主　　题：arrays recording action 

摘      要：Volcano-shaped microelectrodes(nanovolcanoes)functionalized with nanopatterned self-assembled monolayers have recently been demonstrated to report cardiomyocyte action potentials after gaining spontaneous intracellular *** nanovolcanoes exhibit recording characteristics similar to those of state-of-the-art micro-nanoelectrode arrays that use electroporation as an insertion *** this study,we investigated whether the use of electroporation improves the performance of nanovolcano arrays in terms of action potential amplitudes,recording durations,and *** with neonatal rat cardiomyocyte monolayers grown on nanovolcano arrays demonstrated that electroporation pulses with characteristics derived from analytical models increased the efficiency of nanovolcano recordings,as they enabled multiple on-demand registration of intracellular action potentials with amplitudes as high as 62 mV and parallel recordings in up to~76%of the available *** performance of nanovolcanoes showed no dependence on the presence of functionalized nanopatterns,indicating that the tip geometry itself is instrumental for establishing a tight seal at the cell–electrode interface,which ultimately determines the quality of ***,the use of electroporation permitted the recording of attenuated cardiomyocyte action potentials during consecutive days at identical sites,indicating that nanovolcano recordings are nondestructive and permit long-term on-demand recordings from excitable cardiac *** from demonstrating that less complex manufacturing processes can be used for next-generation nanovolcano arrays,the finding that the devices are suitable for performing on-demand recordings of electrical activity from multiple sites of excitable cardiac tissues over extended periods of time opens the possibility of using the devices not only in basic research but also in the context of comprehensive drug testing.