Coxsackievirus B3-induced apoptosis and Caspase-3

作     者：JIAN PING YUAN,WEI ZHAO,HONG TAO WANG,KAI Yu WU,TAO LI,XIAO KUI GUO,SHAN QING TONGDepartment of Microbiology and Parasitology,Shanghai Second Medical University,Shanghai 200025,China 

作者机构：Department of Microbiology and Parasitology Shanghai Second Medical University Shanghai China 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2003年第13卷第3期

页      面：203-209页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

主　　题：Coxsackievirus B3 (CVB3) apoptosis. caspase-3. 

摘      要：Cell death can be classified into two categories: apoptosis and necrosis. Apoptotic pathway can beeither caspase-dependent or caspase-independent. Caspase-independent cytopathic effect (CPE) has beendescribed. In order to evaluate the pattern of HeLa cell death induced by Coxsackievirus B3 (CVB3)and whether apoptosis involves caspase activation, we co-cultivated HeLa cells with CVB3 and detectedthe cytopathic changes, the alteration of mRNA and protein expression of caspase-3 gene plus caspase-3activity, as well as analyzing DNA fragmentation before and after caspase-3 activity inhibition. Accordingto the results, we propose that CVB3 may induce apoptosis and necrosis in HeLa cells, the latter appearingmuch earlier. Caspase-3 is activated at the levels of both transcription and translation, and procaspase-3 isproteolytically cleaved, thus leading to the continuous increasing of both caspase-3 precursor protein and itssubunit. However, besides CPE, apoptosis induced by CVB3 is not a direct consequence of