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Effect of Hyperbaric Oxygen on the Growth of Intracranial Glioma in Rats

Effect of Hyperbaric Oxygen on the Growth of Intracranial Glioma in Rats

作     者:Jian-Bo Ding Jun-Rui Chen Hong-Zhi Xu Zhi-Yong Qin Ding Jian-Bo;Chen Jun-Rui;Xu Hong-Zhi;Qin Zhi-Yong

作者机构:Department of Neurosurgery Huashan Hospital Shanghai Medical College Fudan University Shanghai 200040 China 

出 版 物:《Chinese Medical Journal》 (中华医学杂志(英文版))

年 卷 期:2015年第128卷第23期

页      面:3197-3203页

核心收录:

学科分类:0710[理学-生物学] 03[法学] 07[理学] 08[工学] 09[农学] 0837[工学-安全科学与工程] 071007[理学-遗传学] 0838[工学-公安技术] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 0306[法学-公安学] 

基  金:Acknowledgment We thank the animal facility of the Fudan University for assistance in experiments. Financial support and sponsorship This study was supported by a grant of the National Natural Science Foundation of China (No. 30672153) 

主  题:Angiogenesis Apoptosis Glioma Hyperbaric Oxygen Rat 

摘      要:Background: Numerous studies have confirmed that hyperbaric oxygen (HBO) in combination with radiotherapy or chemotherapy may increase the efficacy of radiotherapy or chemotherapy in patients with glioma. However, whether HBO therapy alone may inhibit or promote the growth of malignant tumors remains controversial. This study aimed to investigate the effect of HBO on the growth of glioma in rats, and the impact of HBO on the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1-alpha (HIF-1α), angiogenesis, and apoptosis of glioma cells. Methods: Male Sprague-Dawley rats were treated with or without HBO after glioma cell inoculation and followed for up to 16 days postinoculation. Rats were randomized to receive bilateral forelimb function tests (n = 20 per group) and head magnetic resonance imaging (n = 5 per group). Differences between HBO and control groups were tested using 2-sample independent t-tests and changes over time within treatment groups were analyzed using a repeated measurement analysis of variance with Bonferroni correction. The effect of H BO on the expression of VEGF, HIF-1α, yon Willebrand factor, angiogenesis, and tumor cell apoptosis were also examined (n = 5 per group). Results: Forelimb function scores were reduced in both HBO-treated and control groups. HBO-treated rats had significantly larger tumor volume and more water in the cerebellum compared with control rats. The intratumoral expression of VEGF was significantly higher in HBO-treated rats compared with control rats (23.2% vs. 13.3%, P = 0.002). HIF-1α was significantly increased in HBO-treated rats compared with controls in the expression of both intratumoral (72.7% vs. 54.9%, P= 0.001) and peritumoral (2.6% vs. 1.9%, P = 0.003) cells. The intratumoral microvessel density (MVD) was significantly higher in the HBO group (15.6 vessels/field vs. 4.4 vessels/field. P 〈 0.001 ), and the peritumoral MVD was not significantly different between the two groups

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