Over-transcription of genes in a parathion-resistant strain of mosquito Culex pipiens quinquefasciatus

作     者：Wei Wang Si-Lu Liu Yang-Yang Liu Chuan-Ling Qiao Shao-Liang Chen Feng Cui 

作者机构：College of Biological Sciences and Technology Beijing Forestry University Beijing State Key Laboratory of Integrated Managementof Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing China 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2015年第22卷第1期

页      面：150-156页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Acknowledgments This study was financially supported by the National Natural Science Foundation of China (Grant No. 31272364) and the Important National Science & Technology Specific Project of China (Grant No. 2012ZX 10004219) 

主　　题：cDNA microarray chymotrypsin gene up-regulation insecticide resistance suppression subtractive hybridization theta glutathione S-transferase 

摘      要：Insecticide resistance is an evolutionary adaptation that develops quite quickly in mosquitoes because of the high selection pressure of chemical insecticides, rapid generation time and large population size. Identification of genes associated with insecticide resistance is fundamental to understand the complex processes responsible for resistance. We compared the gene transcriptional profiles of parathion-resistant and -susceptible Culex pipiens quinquefasciatus using a combination of suppression subtractive hybridization and complementary DNA （cDNA） microarray techniques. A total of 278 colonies were selected from the resistant-susceptible mosquito subtractive library, 38 of which showed more than two fold stronger immunoblotting signals in the resistant strain than in the susceptible strain using cDNA microarray selection. The sequencing results showed that the 38 colonies can be matched to 12 genes of C. p. quinquefasciatus. Eight genes were confirmed to be overexpressed by more than two fold in the resistant strain. These genes encode chymotrypsin-1, theta glutathione S-transferase, lipase 3, larval serum protein 1β chain, cytochrome b, mitochondrial ribosomal large subunit, 28S rRNA, and a protein with unknown function. This study serves as a preliminary attempt to identify new genes associated with organophosphate resistance in this mosquito species and provides insights into the complicated physiological phenomenon of insecticide resistance.