Influence of viscosity on chondrogenic differentiation of mesenchymal stem cells during 3D culture in viscous gelatin solution-embedded hydrogels

作     者：Kyubae Lee Yazhou Chen Xiaomeng Li Naoki Kawazoe Yingnan Yang Guoping Chen Kyubae Lee;Yazhou Chen;Xiaomeng Li;Naoki Kawazoe;Yingnan Yang;Guoping Chen

作者机构：Research Center for Functional MaterialsNational Institute for Materials Science1-1 NamikiTsukubaIbaraki305-0044Japan Department of Materials Science and EngineeringGraduate School of Pure and Applied SciencesUniversity of Tsukuba1-1-1 TennodaiTsukubaIbaraki305-8577Japan School of Mechanics and Engineering ScienceZhengzhou UniversityZhengzhou450001China Graduate School of Life and Environmental ScienceUniversity of Tsukuba1-1-1 TennodaiTsukubaIbaraki305-8571Japan 

出 版 物：《Journal of Materials Science & Technology》 (材料科学技术（英文版）)

年 卷 期：2021年第63卷第4期

页      面：1-8页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 081702[工学-化学工艺] 0806[工学-冶金工程] 0817[工学-化学工程与技术] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0802[工学-机械工程] 0836[工学-生物工程] 0702[理学-物理学] 0801[工学-力学（可授工学、理学学位）] 

基　　金：financially supported by JSPS KAKENHI(Nos.18K19947 18K19945 and 19H04475) 

主　　题：viscosity culture stimulation 

摘      要：Differentiation of human bone marrow-derived mesenchymal stem cells(hMSCs)is regulated by a variety of cues of their surrounding *** particular,mechanical properties of cell culture matrices have been recently disclosed to play a pivotal role in stem cell ***,it remains elusive how viscosity affects the chondrogenic differentiation of hMSCs during three-dimensional(3 D)*** this study,a 3 D culture system that was established by embedding viscous gelatin solution in chemically cross-linked gelatin hydrogels was used for 3 D culture of hMSCs in gelatin solutions with different *** influence of solution viscosity on chondrogenic differentiation of hMSCs was *** gelatin solutions promoted cell proliferation in the order of low,middle and high viscosity while elastic hydrogels restricted cell *** viscosity gelatin solution led to increased production of the cartilaginous *** the synergistic stimulation of chondrogenic induction factors,high viscosity was beneficial for the chondrogenic differentiation of *** results suggested the role of viscosity should be considered as one of the dominant mechanical cues affecting stem cell differentiation.