Resistance genes mediate differential resistance to pine defensive substances α-Pinene and H_(2)O_(2) in Bursaphelenchus xylophilus with different levels of virulence

作     者：Lin Rui Hongbin Liu Rui Liang Xiaoqin Wu Lin Rui;Hongbin Liu;Rui Liang;Xiaoqin Wu

作者机构：Co-Innovation Center for Sustainable Forestry in Southern ChinaCollege of ForestryNanjing Forestry UniversityNanjing 210037JiangsuPeople’s Republic of China Jiangsu Key Laboratory for Prevention and Management of Invasive SpeciesNanjing Forestry UniversityNanjing 210037JiangsuPeople’s Republic of China 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2021年第32卷第4期

页      面：1753-1762页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0907[农学-林学] 0829[工学-林业工程] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 

基　　金：funded partly by the National Key Research and Development Program of China(No.2018YFD0600203) the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) Innovation and Entrepreneurship Training Program for Students of Jiangsu Higher Education Institutions (SPITP) Innovation and Entrepreneurship Training Program for Students of Nanjing Forestry University (No.201710298047Z) 

主　　题：Pine wood nematode Virulence Defensive substances Autophagy gene Detoxification gene Antioxidant gene 

摘      要：The pine wood nematode(PWN),Bursaphelenchus xylophilus(Steiner & Buhrer) Nickle,is the pathogen of pine wilt disease(PWD) which can devastate *** can be of hi gh or low severity and the mechanisms underlying the differences in virulence are ***,it is necessary to study the relationship between differentiation of PWN severity and its resistance to the main defensive substances of pine species(i.e.,α-pinene and H_(2)O_(2)).The feeding rate and fecundity of PWN was examined at different levels of virulence under conditions of a-pinene and H_(2)O_(2) ***,the expression patterns of the main resistance genes of PWN with different virulence were determined under conditions of α-pinene and H_(2)O_(2) *** feeding rate and fecundity of the high virulence strain AMA3 were higher than those of the low virulence strain *** expression levels of the autophagy gene BxATG5,cytochrome P450 gene BxCYP33 D3,and glutathione S-transferase genes BxGST1 and BxGST3 in AMA3 increased significantly upon exposure to α-pinene for 2 h,while these genes showed smaller degrees of upregulation in *** conditions of H_(2)O_(2) stress,the expression levels of BxATG5,catalase genes Bxy-ctl-1 and Bxy-ctl-2,and the 2-cysteine peroxiredoxin gene BxPrx in AMA3 were higher than those in *** findings suggest that high virulence PWN has greater resistance to pine defensive substances α-pinene and H_(2)O_(2) than low virulence PWN,and resistance genes mediate the differential resistance of PWN *** study will contribute to the clarification of the mechanism underlying virulence differentiation of PWN and will advance understanding of the pathogenic mechanism of PWD.