Protective Mechanisms of Suxiao Jiuxin Pills(速效救心丸) on Myocardial Ischemia-Reperfusion Injury in vivo and in vitro

作     者：TAN Ya-fang YU Juan PAN Wen-jun QI Jian-yong ZHANG Min-zhou TAN Ya-fang;YU Juan;PAN Wen-jun;QI Jian-yong;ZHANG Min-zhou

作者机构：AMI Key Laboratory of Chinese Medicine in GuangzhouGuangdong Province Hospital of Chinese Medicinethe 2nd Affiliated Hospital of Guangzhou University of Chinese MedicineGuangdong Provincial Academy of Chinese Medical ScienceGuangzhou 510006China Intensive Care Research Team of Traditional Chinese MedicineGuangdong Province Hospital of Chinese Medicinethe 2nd Affiliated Hospital of Guangzhou University of Chinese MedicineGuangdong Provincial Academy of Chinese Medical ScienceGuangzhou 510006China Animal LaboratoryGuangdong Province Hospital of Chinese MedicineGuangzhou 510006China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2020年第26卷第8期

页      面：583-590页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 10[医学] 100602[医学-中西医结合临床] 

基　　金：Supported by the National Natural Science Foundation of China(No.81473471 and No.81603429) Foundation of Guangdong Hospital of Chinese Medicine(No.YK2013B2N11,No.YN2014ZH01,No.YN2014ZHR203,and No.YN2016QJ19)。 

主　　题：myocardial ischemia and reperfusion injury Suxiao Jiuxin Pills GATA4 Chinese medicine mouse 

摘      要：Objective:To study the protective mechanism of Chinese medicine Suxiao Jiuxin Pills(速效救心丸,SXJ)on myocardial ischemia and reperfusion(I/R)injury.Methods:Mouse myocardial I/R injury model was created by 30-min coronary artery occlusion followed by 24-h reperfusion,the mice were then divided into the sham group(n=7),the I/R group(n=13),the tirofiban group(TIR,positive drug treatment,n=9),and the SXJ group(n=11).Infarct size(IS),risk region(RR),and left ventricle(LV)were analyzed with double staining methods.In addition,H9C2 rat cardiomyocytes were cultured with Na2S2O4 to simulate I/R in vitro.The phosphorylation of extracellular regulated protein kinases1/2(ERK1/2),protein kinase B(AKT),glycogen synthase kinase-3β(GSK3β),and protein expression of GATA4 in nucleus were detected with Western blot assay.Results:The ratio of IS/RR in SXJ and TIR groups were lower than that in I/R group(SXJ,22.4%±6.6%;TIR,20.8%±3.3%;vs.I/R,35.4%±3.7%,P0.05,respectively).In vitro experiments showed that SXJ increased the Na2S2O4-enhanced phosphorylation of AKT/GSK3βand nuclear expression of GATA4.Conclusion:SXJ prevents myocardial I/R injury in mice by activating AKT/GSK3βand GATA4 signaling pathways.