根癌农杆菌介导的反义VeFAD_2基因对少根根霉的遗传转化及其影响因子
Genetic Transformation in Rhizopus arrhizus of Antisense VeFAD_2 Gene via Agrobacterium tumefaciens-mediated Transformation and Its Influencing Factors作者机构：中国林业科学研究院亚热带林业研究所浙江富阳311400
出 版 物：《林业科学研究》 (Forest Research)
年 卷 期：2010年第23卷第4期
页 面：592-596页
核心收录：
学科分类：0907[农学-林学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 090102[农学-作物遗传育种] 0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 090701[农学-林木遗传育种] 0829[工学-林业工程] 0901[农学-作物学] 0902[农学-园艺学] 0836[工学-生物工程] 0713[理学-生态学]
基 金：浙江省科技厅重大项目(2006C12030 2005C12003)
摘 要：Agrobacteriurn tumefaciens-mediated transformation(AtMT) method was used to study the genetic transformation in Rhizopus arrhizus of antisense expression vector pBI121-fad2 of VeFAD2 *** studied the key factors influencing transformation,such as *** strains,bacterial cell volume initially used,co-cultivation time,effective period and concentration of *** results shows that the ideal *** strain is AGL-1,the optimum bacterial cell volume initially used is 50-100 μL and co-culture 24 hours will get the most *** co-culture period,AS induction is *** is beneficial to improve transformation ratio by adding to 200 μmol·L-1 AS at preincubate period and increasing AS concentration to 400-600 μmol·L-1 at co-culture *** determined that antisense VeFAD2 gene has been integrated into the genome of *** by PCR-Southern detection.
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