Preparation and Inhibitory Effects of 20(S)-Ginsenoside Rh_2 on Hep-A-22 Cells

作     者：ZHOU Hong-yu JIN Yong-ri WEI Wei SHI Xiao-lei YANG Rui-jie YANG Shi-jie LI Xu-wen 

作者机构：College of Chemistry Jilin Uuniversity Changchun 130012 P. R. China Basic Medical School Jilin Uuniversity Changchun 130021 P. R. China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2010年第26卷第4期

页      面：567-571页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 071007[理学-遗传学] 070303[理学-有机化学] 0901[农学-作物学] 0703[理学-化学] 090102[农学-作物遗传育种] 

基　　金：Supported by the National Natural Science Foundation of China(No.30672654) 

主　　题：20(S)-Ginsenoside Rh2 Preparation Alkaline-degradation Hep-A-22 cell Inhibitory effect 

摘      要：A modified method of preparing 20（S）-ginsenoside Rh2（G-Rh2） and the inhibitory effect of 20（S）-ginsenoside Rh2 on Hep-A-22 cells were investigated. The total saponins and strong alkali were dissolved in glycerol at the atmospheric pressure, and the degradation was performed at a high temperature. After G-Rh2 had been isolated and purified, MTT（methyl thiazolyl tetrazolium） assay was applied to evaluating the effect of 20（S）-ginsenoside Rh2 on the cells viability and morphological changes were observed. It was shown that 20（S）-ginsenoside Rh2 can reduce Hep-A-22 cells viability in dose-dependent manner and the cells took on cell shrinkage, membrane blebbing, chromosomal condensations, especially under the higher concentrations of it. In conclusion, 20（S）-ginsenoside Rh2 can be prepared effectively that not only decreases viability but also induces the apoptosis of Hep-A-22 cells.