Shenkang Ⅶ Recipe Attenuates Unilateral Ureteral Obstruction-induced Renal Fibrosis via TGF-β/Smad, NF-κB and SHH Signaling Pathway
作者机构：Hubei University of Chinese MedicineWuhan 430065China Hubei Provincial Hospital of Traditional Chinese MedicineWuhan 430061China Hubei Provincial Academy of Traditional Chinese MedicineWuhan 430061China
出 版 物：《Current Medical Science》 (当代医学科学（英文）)
年 卷 期：2020年第40卷第5期
页 面：917-930页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：This study was supported by Academic Experience Inheritance of the Sixth National Group of Old Chinese Medicine Experts of the State Administration of Traditional Chinese Medicine(No.2017) the key projects of Hubei Provincial Department of Health(No.JX6A09)
主 题：ShenkangⅦrecipe(SK-7) chronic kidney disease renal fibrosis signaling pathway
摘 要：This study aimed to explore the protective effects of the traditional Chinese Medicine formula Shenkang Ⅶ recipe(SK-7)on renal fibrosis and the *** fibrosis was induced by unilateral ureteral obstruction(UUO)in *** rats were then divided into 5 groups:control group(Sham operation),UUO model group,UUO model plus low to high doses of SK-7(0.5,1.0,or 2.0 g/kg/day,for 14 days)*** animals were sacrificed on the 7th or 14th day.K idney tissues were collected for histopathological exarminations(hematoxylin and cosin and Masson s trichrome staining).Immunohistochemistry was used to detect the expression of collagen type II(Col II),fibronectin(FN),α-smooth muscle actin(a-SMA),TIMP metallopeptidase inhibitor 2(TIMP2),matrix metallopeptidase 2(MMP2),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and monocyte chemotactic protein-1(MCP-1).The TGF-β1/Smad,NF-κB and Sonic hedgehog signaling proteins were detected by Western *** results showed that SK-7 prevented UUO-induced renal injury and accumulation of collagen *** fbrosis biomarkers Col Ⅲ,FN,α-SMA and TMP2 were increased in the rats after UUO and decreased by SK-7,while MMP2 was upregulated after ***-7 also suppressed the levels of TNF-α,IL-1βand MCP-1 in UUO *** addition,SK-7 inhibited activation of the TGF-B/Smad,NF-κB and sonic hedgehog signaling(SHH)*** together,these findings suggest that SK-7 may regulate the synthesis and degradation of extracellular matrix,reduce inflammation and suppress the proliferation of fibroblasts,by blocking the TGF-β1/Smad,NF-κB and SHH signaling pathways to exert its anti-renal fbrosis effect in UUO rats.
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