Protective effect of Yi-Qi-Huo-Xue Decoction against ischemic heart disease by regulating cardiac lipid metabolism

作     者：LI Fang-He HUANG Xiao-Lou WANG Hui GUO Shu-Wen LI Ping LI Fang-He;HUANG Xiao-Lou;WANG Hui;GUO Shu-Wen;LI Ping

作者机构：The Third Affiliated HospitalBeijing University of Chinese MedicineBeijing 100029China School of Traditional Chinese MedicineBeijing University of Chinese MedicineBeijing 100029China College of Acupuncture and OrthopedicsGuizhou University of Traditional Chinese MedicineGuiyang 550025China Fangshan HospitalBeijing University of Chinese MedicineBeijing 102400China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2020年第18卷第10期

页      面：779-792页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Natural Science Foundation of China(No.81473552) the China Postdoctoral Science Foundation(No.2019TQ0043) 

主　　题：Yi-Qi-Huo-Xue Decoction Lipid metabolism Ischemic heart disease AMPK-dependent pathway Fatty acid oxidation 

摘      要：Yi-Qi-Huo-Xue Decoction(YQHX)is the recombination of Dang-Gui-Bu-Xue Decoction(DBD),which is one of the well-known traditional Chinese Medicine(TCM)prescription,and has long been shown to have significant protective effects against myocardial ischemic *** previous studies,we found that YQHX could regulate lipid and glucose metabolism,promote angiogenesis,attenuate inflammatory response,and ameliorate left ventricular function in myocardial ischemia rat ***,the underlying mechanism of how YQHX involves in lipid metabolism remains unclear so *** this study,the underlying mechanism of YQHX in lipid metabolism disorders was elucidated in a myocardial ischemia rat model and a hypoxia-induced H9 c2 cell injury ***(8.2 g·kg-1)and positive-control drug trimetazidine(10 mg·kg-1)were administered daily on the second day after left anterior descending(LAD)*** 7 days and 28 days after surgery,changes of cardiac morphology,structure,and function were evaluated by H&E staining and echocardiography,*** plasma lipid levels and mitochondrial ATP content were also *** blot and RT-PCR were used to determine the protein and mRNA expressions of AMPK,PGC-1α,CPT-1α,and PPARα.YQHX improved cardiac function and ameliorated lipid metabolism ***,YQHX increased the expression of p-AMPK,PGC-1α,and CPT-1αwithout changing PPARαin ischemic rat *** vitro,YQHX activated the protein and mRNA expression of PGC-1α,CPT-1α,and PPARαin hypoxia-induced H9 c2 cells injury,whereas AMPK inhibitor Compound c blocked the effects of *** together,the results suggest that YQHX reduces lipid metabolism disorders in myocardial ischemia via the AMPK-dependent signaling pathway.