Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System

作     者：WANG Ke YONG Yue ZHOU Jia ZHOU Wen-xiong GUO Jun CHEN Tong-yu WANG Ke;YONG Yue;ZHOU Jia;ZHOU Wen-xiong;GUO Jun;CHEN Tong-yu

作者机构：Department of Cardiothoracic SurgeryYueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghai(200437)China Institute of Clinical ImmunologyYueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghai(200437)China Research Institute of Acupuncture AnesthesiaShuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai(201203)China Department of AnesthesiologyShuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai(201203)China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2021年第27卷第2期

页      面：98-105页

核心收录：

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China(No.81573796,81673756,and 81503395) Medical Guidance Project of the Shanghai Science and Technology Commission(No.16401932400) research grant from Shanghai Hospital Development Center(No.16CR1031B) The Interdisciplinary Project of"Clinical Immunology of Traditional Chinese Medicine"in Shanghai(No.30304113598) 

主　　题：electroacupuncture Chinese medicine immunosuppression opioid receptor T-lymphocytes 

摘      要：Objective:To investigate the action mechanisms of electroacupuncture(EA)on postoperative ***:Male C57 BL/6 mice(5–7 weeks old)were randomly divided into:the sham injury group,the surgical trauma stressed group,the EA group[surgery+2/100 Hz EA at Neiguan(PC 6)],and the EA+Nal(surgery+EA+intraperitoneal injection of naloxone).Abdominal surgical trauma stress mice model was *** was performed on bilateral PC 6 acupoints by an EA apparatus(2/100 Hz)for 20 min once a day for 3 *** m RNA expressions of MOR,DOR,and KOR in thymus and L3-5 dorsal root ganglions(DRG)were determined by quantitative real-time polymerase chain reaction(q RT-PCR)and the protein expressions of MOR,DOR,and KOR in thymus were measured by Western *** cytometry assay was used to detect the levels of T lymphocyte subtypes in the peripheral ***:Surgical trauma induced decreased the m RNA expression level of MOR in both thymus(P0.05).Furthermore,T lymphocyte population of CD3^+ and CD4^+ was decreased in the peripheral blood after surgical trauma(both P0.01).EA treatment can significantly elevate the population of CD3^+(P0.01),CD4^+(P0.05)and CD8^+T cells(P0.01).Intraperitoneal injection of the non-selective opioid receptor antagonist naloxone blocked the up-regulation of T lymphocytes by ***:EA may improve postoperative immunosuppression through the peripheral opioid system.