Metastatic status of sentinel lymph nodes in breast cancer determined with photoacoustic microscopy via dual-targeting nanoparticles
作者机构：Britton Chance Center and MOE Key Laboratory for Biomedical PhotonicsSchool of Engineering SciencesWuhan National Laboratory for Optoelectronics–Huazhong University of Science and TechnologyWuhanHubei 430074China School of Biomedical EngineeringHainan UniversityHaikouHainan 570228China
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2020年第9卷第1期
页 面：450-465页
核心收录：
学科分类：0808[工学-电气工程] 1002[医学-临床医学] 0809[工学-电子科学与技术（可授工学、理学学位）] 0805[工学-材料科学与工程（可授工学、理学学位）] 100214[医学-肿瘤学] 0702[理学-物理学] 10[医学]
基 金：supported by the National Science Fund for Distinguished Young Scholars(81625012) the National Natural Science Foundation of China(61721092) the Fundamental Research Funds for the Central Universities(2019kfyXMBZ022) Programme for HUST Academic Frontier Youth Team(Zhang,Z.H.) the Innovation Fund of WNLO
摘 要：Detection of sentinel lymph nodes(SLNs)is critical to guide the treatment of breast ***,distinguishing metastatic SLNs from normal and inflamed lymph nodes(LNs)during surgical resection remains a ***,we report a CD44 and scavenger receptor class B1 dual-targeting hyaluronic acid nanoparticle(5K-HA-HPPS)loaded with the near-infra-red fluorescent dye DiR-BOA for SLN imaging in breast *** small sized(~40 nm)self-assembled 5K-HA-HPPSs accumulated rapidly in the SLNs after intradermal *** with normal popliteal LNs(N-LN),there were ~3.2-fold and ~2.4-fold increases in fluorescence intensity in tumour metastatic SLNs(T-MLN)and inflamed LNs(Inf-LN),respectively,6 h after nanoparticle *** importantly,photoacoustic microscopy(PAM)of 5KHA-HPPS showed a significantly distinct distribution in T-MLN compared with N-LN and *** were mainly distributed at the centre of T-MLN but at the periphery of N-LN and *** ratio of PA intensity(R)at the centre of the LNs compared with that at the periphery was 5.93±0.75 for T-MLNs of the 5K-HA-HPPS group,which was much higher than that for the Inf-LNs(R=0.2±0.07)and N-LNs(R=0.45±0.09).These results suggest that 5K-HA-HPPS injection combined with PAM provides a powerful tool for distinguishing metastatic SLNs from pLNs and inflamed LNs,thus guiding the removal of SLNs during breast cancer surgery.
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