Detection of QTL and QTN and candidate genes for oil content in soybean using a combination of four-way-RIL and germplasm populations

作     者：Xiyu Li Kaixin Zhang Xu Sun Shanshan Huang Jiajing Wang Chang Yang Mahfishan Siyal Chao Wang Chunlan Guo Xiping Hu Wen-Xia Li Hailong Ning Xiyu Li;Kaixin Zhang;Xu Sun;Shanshan Huang;Jiajing Wang;Chang Yang;Mahfishan Siyal;Chao Wang;Chunlan Guo;Xiping Hu;Wen-Xia Li;Hailong Ning

作者机构：Key Laboratory of Soybean BiologyMinistry of Education/Key Laboratory of Soybean Biology and Breeding/GeneticsMinistry of AgricultureNortheast Agricultural UniversityHarbin 150030HeilongjiangChina Yancheng Institute of TechnologyYancheng 224051JiangsuChina Key Laboratory of Crop Biotechnology Breeding of the Ministry of AgricultureBeidahuang Kenfeng Seed Co.Ltd.Harbin 150030HeilongjiangChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2020年第8卷第5期

页      面：802-811页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：This research was financially supported by Hundredthousand and Million Project of Heilongjiang Province for Engineering and Technology Science(2019ZX16B01) We thank Professor Jiankang Wang(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences)for valuable suggestions 

主　　题：soybean germplasm planted 

摘      要：Soybean is a source of edible oil for humans and provides a third of the vegetable oil consumed *** seed oil content in seeds is thus a key objective in soybean *** the present study,a four-way recombinant inbred line(FW-RIL)population comprising 144 lines,planted in 10 environments,and a germplasm panel of 455 accessions,planted in two environments,were used to collect oil-content ***,59 quantitative trait loci(QTL)were detected in the FW-RIL population by inclusive complete interval mapping on a linkage map consisting of 2232 single-nucleotide polymorphism(SNP)*** in the FW-RILs,44 quantitative trait nucleotides(QTNs)were detected by association analysis using 109,676 SNP markers and fivemethods of multi-locus genome-wide association ***,77 QTN were detected by association analysis in the germplasm panel using 63,306 *** of the QTL and QTN suggested four QTN controlling oil *** analysis was performed on genes in attenuation regions of these four QTN,and two candidate genes involved in the synthesis or metabolism of soybean oil were *** findings provide useful information about the genetics of oil content and may contribute to its genetic improvement by marker-assisted selection.