酒精性肝损伤与小肠通透性改变的关系及中药的干预作用

作     者：胡义扬 方志红 崔剑巍 王晓柠 彭景华 冯琴 张慧 HU Yiyang;FANG Zhihong;CUI Jianwei;WANG Xiaoning;PENG Jinhua;FENG Qin;ZHANG Hui

作者机构：上海中医药大学肝病研究所上海201203 

出 版 物：《科学技术与工程》 (Science Technology and Engineering)

年 卷 期：2005年第5卷第22期

页      面：1770-1771,1776页

学科分类：100506[医学-中医内科学] 1006[医学-中西医结合] 1005[医学-中医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：国家自然科学基金(30371818)资助 

主　　题：酒精 肝损伤 内毒素 小肠通透性 中药 

摘      要：To investigate the effects and to elucidate the mechanism of Chinese herbs Recipe(consisting of Rhizoma Atractylodis Macrocephalae, Radix Salviae Simplicifoliae, Fructus Aurantii, et al) on antagonizing alcoholic liver injury from the pathological relationship between alcoholic liver injury, gut permeability and endotoxin leakage. The SD rats were fed the Lieber- Decarli liquid alcohol die(t35% of calories from alcohol)to deveclp the alcoholic liver injury model. 42 male SD rats were divided into 4 groups:normal group(n=6),no- alcohol liquid diet group in which alcohol was replaced with carbohydrates(Control group, n=12), alcohol liquid diet group(alcohol group, n=12) and alcohol liquid eiet with Chinese herbs group(herbs group, n=12). Starting from the third week, the rats were fed distilled water, no- alcohol liquid diet, alcohol liquid diet or alcoholic liquid diet with Chinese herbs by intragastric infusion for 6 weeks. Then the rats were fast for 5 h. After that, each group received 10 mg/kg of endotoxin(lipidopolysacchride, LPS)by intragastric infusion. 3.5 hours later, the blood from portal vein and vena cava was harvested and liver biopsy was performed to analyze:1. The histopathological change in small intestine (HE staining and electron microscopic observation); 2. The histopathological change in liver(HE staining, oil red O staining); 3. The TG and r- GT activities in liver and the ALT, AST activities in serum; 4. The plasma endotoxin level in portal vein blood; 5. The protein expressions(on Wetern blot) of P- I k B and TNF- a in ***: that It is *** degeneration was seen in over 1/3 of the heptocytes in the alcohol group. The liver TG level in this group was about 7.3 times higher than that in the normal group and 4.9 times higher than that in the control group. In addition, the serum ALT, AST activities as well as liver r- GT activity in the alcohol group were significantly elevated than those in the normal and control group. Observation un