Does acupuncture response increase with the increasing dosage:A preclinical study investigating rats with sleep deprivation

作     者：Fei-yi ZHAO Yan XU Guo-hua WANG Qia-yi MA Qiang-qiang FU Zhi-min LI Hong XU Li-ping YUE 赵非一;徐燕;王国华;马恰怡;岳立萍;许红;李志敏;付强强

作者机构：Department of NursingSchool of International Medical TechnologyShanghai Sanda UniversityShanghai 201209China Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 200071China Shanghai Tianshan Hospital of Traditional Chinese MedicineShanghai 200051China Research DepartmentYangpu hospitalTongji University School of MedicineShanghai 200090China 

出 版 物：《World Journal of Acupuncture-Moxibustion》 (世界针灸杂志（英文版）)

年 卷 期：2020年第30卷第3期

页      面：207-216页

核心收录：

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：Natural Science Foundation of Shanghai:No.17ZR1428100 TCM Science and Technology Innovation Project of Shanghai Health and Family Planning Commission:No.ZYKC20161016 Special Project for Clinical Research Shanghai Municipal Health Commission:No.20174Y0009 

主　　题：Electroacupuncture Sleep deprivation Depressive-like behaviors Anxiety-like behaviors Behavioral test 

摘      要：Background:The effectiveness of acupuncture for insomnia and insomnia-related anxiety and depression has been widely investigated in clinical ***,whether higher dosage(more frequent)acupuncture can bring greater responses(a greater size of effect)is less ***:This study is designed to investigate whether a five-times weekly(5 Ts/w)electroacupuncture(EA)treatment is better than a three-times weekly(3 Ts/w)EA in alleviating sleep deprivation,and sleep disturbance-induced cognitive dysfunctions and negative emotions in rats through four various behavioral te ***:Forty-six male Sprague-Dawley rats were randomly divided into control group(n=10),model group(n=12),EA-3 Ts/w group(n=12),and EA-5 Ts/w group(n=12).Except for the control group,the other three groups were established as chronic sleep deprivation models via the modified multi-platform water environment ***,rats in both EA-3 Ts/w group and EA-5 Ts/w group received corresponding dosage of EA therapy,*** modeling and interventions,all four groups received four behavioral tests as follows:(1)sleep behavioral monitoring and evaluation was achieved by Comprehensive Lab Animal Monitoring System(CLAMS).(2)Cognitive functions were assessed by Novel Object Recognition(NOR)test.(3)Depressive-like behaviors was evaluated by Open-Field(OF)test.(4)Anxietylike behaviors was appraised by Elevated Plus-Maze(EPM)*** finishing the behavioral tests,the hippocampus of each rat was removed and its synaptic structure changes were observed under electron ***:(1)CLAMS:two EA groups derived more sleep time within 24 h than the model group(both P0.05).(2)NOR test:NOR ratio in the EA-3 Ts/w group was higher than that of the model group(P0.05)but lower than that of either the control group(P0.05)or the EA-5 Ts/w group(P0.05).(3)OF test:the difference of horizontal movements bet