Physical understanding of axonal growth patterns on grooved substrates:groove ridge crossing versus longitudinal alignment

作     者：Deming Zhang Hairui Suo Jin Qian Jun Yin Jianzhong Fu Yong Huang Deming Zhang;Hairui Suo;Jin Qian;Jun Yin;Jianzhong Fu;Yong Huang

作者机构：The State Key Laboratory of Fluid Power and Mechatronic SystemsSchool of Mechanical EngineeringZhejiang UniversityHangzhou 310028China Key Laboratory of 3D Printing Process and Equipment of Zhejiang ProvinceSchool of Mechanical EngineeringZhejiang UniversityHangzhou 310028China School of AutomationHangzhou Dianzi UniversityHangzhou 310018China Key Laboratory of Soft Machines and Smart Devices of Zhejiang ProvinceDepartment of Engineering MechanicsZhejiang UniversityHangzhou 310027China Department of Mechanical and Aerospace EngineeringUniversity of FloridaGainesvilleFL 32611USA 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2020年第3卷第4期

页      面：348-360页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 0817[工学-化学工程与技术] 08[工学] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 0836[工学-生物工程] 0811[工学-控制科学与工程] 

基　　金：This study was partially supported by the National Key Research and Development Program of China(Grant No.2018YFA0703000) the Key Research and Development Program of Zhejiang Province(Grant No.2017C01063) the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(Grant No.51821093) the National Natural Science Foundation of China(Grant Nos.U1609207,11672268) ZJU Scholarship for Outstanding Doctoral Candidates and Scholarship Program supported by China Scholarship Council(No.201906320187) 

主　　题：Grooved substrates Neuron outgrowth Axonal outgrowth model Axonal crossing 

摘      要：Surface topographies such as micrometric edges and grooves have been widely used to improve neuron ***,finding the mechanism of neuron–surface interactions on grooved substrates remains a *** this work,PC12 cells and chick forebrain neurons(CFNs)were cultured on grooved and smooth polyacrylonitrile *** was found that CFNs showed a tendency of growing across groove ridges;while PC12 cells were only observed to grow in the longitudinal direction of *** further investigate these observations,a 3D physical model of axonal outgrowth was *** this model,axon shafts are simulated as elastic 3D beams,accounting for the axon outgrowth as well as the focal contacts between axons and ***,the bending direction of axon tips during groove ridge crossing is governed by the energy minimization *** physical model predicts that axonal groove ridge crossing is contributed by the bending compliance of axons,caused by lower Young’s modulus and smaller *** work will aid the understanding of the mechanisms involved in axonal alignment and elongation of neurons guided by grooved substrates,and the obtained insights can be used to enhance the design of instructive scaffolds for nerve tissue engineering and regeneration applications.