Human embryonic stem cell-derived mesenchymal stem cells improved premature ovarian failure

作     者：Khadijeh Bahrehbar Mojtaba Rezazadeh Valojerdi Fereshteh Esfandiari Rouhollah Fathi Seyedeh-NafisehHassani Hossein Baharvand 

作者机构：Department of Stem Cells and Developmental BiologyCell Science Research CenterRoyan Institute for Stem Cell Biology and TechnologyTehran ***Iran Department of Developmental BiologyUniversity of Science and CultureTehran ***Iran Department of EmbryologyReproductive Biomedicine Research CenterRoyan Institute for Reproductive BiomedicineTehran ***Iran Department of AnatomyFaculty of Medical ScienceTarbiat Modares UniversityTehran ***Iran 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2020年第12卷第8期

页      面：857-878页

核心收录：

学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学] 

基　　金：Ministry of Health and Medical Education, MOHME, (700/147) Royan Institute National Institute for Medical Research Development, NIMAD, (963255) 

主　　题：Premature ovarian failure Human embryonic stem cells Chemotherapy drugs Mesenchymal stem cell Bone marrow Apoptosis 

摘      要：BACKGROUND Premature ovarian failure(POF)affects many adult women less than 40 years of age and leads to *** to previous reports,various tissue-specific stem cells can restore ovarian function and folliculogenesis in mice with chemotherapy-induced *** embryonic stem cells(ES)provide an alternative source for mesenchymal stem cells(MSCs)because of their similarities in phenotype and immunomodulatory and anti-inflammatory *** stem cell-derived mesenchymal stem cells(ES-MSCs)are attractive candidates for regenerative medicine because of their high proliferation and lack of barriers for harvesting tissue-specific ***,possible therapeutic effects and underlying mechanisms of transplanted ES-MSCs on cyclophosphamide and busulfan-induced mouse ovarian damage have not been *** To evaluate ES-MSCs vs bone marrow-derived mesenchymal stem cells(BMMSCs)in restoring ovarian function in a mouse model of chemotherapy-induced premature ovarian *** Female mice received intraperitoneal injections of different doses of cyclophosphamide and busulfan to induce *** human ES-MSCs or BMMSCs were transplanted into these *** days after the mice were injected with cyclophosphamide and busulfan and 4 wk after transplantation of the ESMSCs and/or BM-MSCs,we evaluated body weight,estrous cyclicity,folliclestimulating hormone and estradiol hormone concentrations and follicle count were used to evaluate the POF model and cell ***,terminal deoxynucleotidyl transferase mediated 2-deoxyuridine 5-triphosphate nick end labeling,real-time PCR,Western blot analysis and immunohistochemistry and mating was used to evaluate cell ***-linked immunosorbent assay was used to analyze vascular endothelial growth factor,insulin-like growth factor 2 and hepatocyte growth factor levels in ES-MSC condition medium in order to investigate the mechanisms that underlie their *** Th