Practical choice for robust and efficient differentiation of human pluripotent stem cells
作者机构：Institute of Regenerative MedicineAffiliated Hospital of Jiangsu UniversityJiangsu UniversityZhenjiang 212001Jiangsu ProvinceChina School of Biotechnology and Heath SciencesWuyi UniversityJiangmen 529020Guangdong ProvinceChina Department of Gastrointestinal and Hepato-Biliary-Pancreatic SurgeryUniversity of Tsukuba Faculty of MedicineTsukubaIbaraki 305-8575Japan City University School of MedicineYokohamaKanagawa 234-0006Japan Division of Regenerative MedicineCenter for Stem Cell Biology and Regenerative MedicineThe Institute of Medical Sciencethe University of TokyoTokyo 108-8639Japan
出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)
年 卷 期：2020年第12卷第8期
页 面：752-760页
核心收录：
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学]
基 金：Ministry of Education, Culture, Sports, Science and Technology, MEXT, (18H02866) Ministry of Education, Culture, Sports, Science and Technology, MEXT National Natural Science Foundation of China, NSFC, (81770621) National Natural Science Foundation of China, NSFC Natural Science Foundation of Jiangsu Province, (BK20180281) Natural Science Foundation of Jiangsu Province
主 题：Human pluripotent stem cells Three dimensional Embryoid body Differentiation Efficient Three germ layers
摘 要：Human pluripotent stem cells(hPSCs)have the distinct advantage of being able to differentiate into cells of all three germ *** cells or tissues derived from hPSCs have many uses such as drug screening,disease modeling,and transplantation *** are currently a wide variety of differentiation methods ***,most of the existing differentiation methods are unreliable,with uneven differentiation efficiency and poor *** the same time,it is difficult to choose the optimal method when faced with so many differentiation schemes,and it is time-consuming and costly to explore a new differentiation ***,it is critical to design a robust and efficient method of *** this review article,we summarize a comprehensive approach in which hPSCs are differentiated into target cells or organoids including brain,liver,blood,melanocytes,and mesenchymal *** was accomplished by employing an embryoid body-based three-dimensional(3D)suspension culture system with multiple cells *** method has high stable differentiation efficiency compared to the conventional 2D culture and can meet the requirements of clinical ***,ex vivo co-culture models might be able to constitute organoids that are highly similar or mimic human organs for potential organ transplantation in the future.
维普期刊数据库