Validation of Bacteroidales-based microbial source tracking markers for pig fecal pollution and their application in two rivers of North China
作者机构：College of Resources and EnvironmentUniversity of Chinese Academy of SciencesBeijing 100049China
出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)
年 卷 期：2020年第14卷第4期
页 面：139-146页
核心收录：
学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 0815[工学-水利工程]
基 金：This work was supported by the National Key Research and Development Program of China(No.2016YFC0503601) Excellent Young Teachers'Scientific Research Ability Improvement Project of University of Chinese Academy of Sciences(No.Y95401FXX2)
主 题：Microbial source tracking Pig fecal pollution 16S rRNA genem arkers Pig-specific Bacteroidales
摘 要：In China,pig feces is the predominant source of excrement produced b y animal *** use or direct discharge of pig feces can result in contamination of natural water *** source tracking(MST)technology can identify the sources of fecal pollution in environmental water,and contribute to the management of pig fecal pollution by local environmental protection ***,the accuracy of such assays can be context-dependent,and they have not been comprehensively evaluated under Chinese *** aimed to compare the performance of five previously reported nig-specific MST assays(PF,Pig-Bac 1 STBR,Pig-Bac2 SYBR,Pig-1-Bac TaqMan,and Pig-2-Bac TaqMan,which are based on Bacteroidales 16S rRNA gene markers)and apply them in two rivers of North *** collected a total of 173 fecal samples from pigs,cows,goats,chickens,humans,and horses across *** PF assay optimized in this study showed outstanding qualitative performance and achieved 100%specificity and ***,the two SYBR green qPCR assays(Pig-Bac1 SYBR and Pig-Bac2 SYBR)cross-reacted with most non-pig fecal *** contrast,both the Pig-l-Bac TaqMan and Pig-2-Bacr TaqMan assays gave 100%specificity and *** these,the Pig-2-Bac TaqMan assay showed higher *** results regarding the specificity of these pig-specific MST assays differ from those reported in Thailand,Japan,and *** the PF and Pig-2-Bac TaqMan assays,a field test comparing the levels of pig fecal pollution in rivers near a pig farm before and after comprehensive environmental pollution governance indicated that pig fecal pollution was effectively controlled at this location.
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