Antitubercular activity of the semi-polar extractives of Uvaria rufa

作     者：Allan Patrick G Macabeo Florie A Tudla Karsten Krohn Scott G Franzblau 

作者机构：Organic Synthesis and Phytochemistry LaboratoryResearch Center for the Natural SciencesUniversity of Santo TomasEspana Blvd.Manila 1015Philippines Institut fr Organische ChemieUniversitat RegensburgUniversitatsstrasse 3193053 RegensburgGermany Institut fr Organische ChemieUniversitat PaderbornGermany Institute for Tuberculosis ResearchCollege of PharmacyUniversity of Illinois.IL 60085USA 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2012年第5卷第10期

页      面：777-780页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Uvaria rufa Antitubercular Mycobacterium tuberculosis H_(37)Rv 

摘      要：Objective:To investigate the inhibitory activity of the chloroform extract,petroleum ether and chloroform sub-extracts,lead-acetate treated chloroform extract,fractions and secondary metabolites of Uvaria rufa(***) against Mycobacterium tuberculosis(***) H_(37)Rv. Methods:The antituberculosis susceptibility assay was earried out using the colorimetric Microplate Alamar blue assay(MABA).In addition,the cytotoxicity of the most active fraction was evaluated using the VERO cell toxicity ***:The in vitro inhibitory activity against *** H_(37)Rv increased as purification progressed to fractionation(MIC up to 23μg/mL). The chloroform extract and its sub-extracts showed moderate toxicity while the most active fraction from chloroform sub-extract exhibited no cytotoxicity against VERO ***, the lead acetate-treated crude chloroform extract and its fractions showed complete inhibitions (100%) with MIC values up to 8μg/*** screening of the most active fraction showed,in general,the presence of terpenoids,steroids and phenolic *** of the antimycobacterial activity of known secondary metabolites isolated showed no promising inhibitory activity against the test ***:The present results demonstrate the potential of *** as a phytomedicinal source of compounds that may exhibit promising antituberculosis *** addition,elimination of polar pigments revealed enhanced inhibition against *** H_(37)*** several compounds known for this plant did not show antimycobacterial activity,the obtained results are considered sufficient reason for further study to isolate the metabolites from *** responsible for the antitubercular activity.