Porcine Immunoglobulin Fc Fused P30/P54 Protein of African Swine Fever Virus Displaying on Surface of S. cerevisiae Elicit Strong Antibody Production in Swine

作     者：Chen Chen Deping Hua Jingxuan Shi Zheng Tan Min Zhu Kun Tan Lilin Zhang Jinhai Huang Chen Chen;Deping Hua;Jingxuan Shi;Zheng Tan;Min Zhu;Kun Tan;Lilin Zhang;Jinhai Huang

作者机构：School of Life SciencesTianjin UniversityTianjin 300072China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2021年第36卷第2期

页      面：207-219页

核心收录：

学科分类：090602[农学-预防兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the National Key Research and Development Program of China (2018YFD0500500) 

主　　题：African swine fever virus(ASFV) S.cerevisiae Porcine immunoglobulin Fc P30-Fcγ/P54-Fcαfusion proteins 

摘      要：African swine fever virus(ASFV)infects domestic pigs and European wild boars with strong,hemorrhagic and high *** primary cellular targets of ASFV is the porcine *** to now,no commercial vaccine or effective treatment available to control the *** this study,three recombinant Saccharomyces cerevisiae(***)strains expressing fused ASFV proteins-porcine Ig heavy chains were constructed and the immunogenicity of the ***-vectored cocktail ASFV feeding vaccine was further *** be specific,the P30-Fcγand P54-Fcαfusion proteins displaying on surface of *** cells were produced by fusing the Fc fragment of porcine immunoglobulin IgG1 or IgA1 with p30 or p54 gene of ASFV *** recombinant P30-Fcγand P54-Fcαfusion proteins expressed by *** were verified by Western blotting,flow cytometry and immunofluorescence *** immunoglobulin Fc fragment fused P30/P54 proteins elicited P30/P54-specific antibody production and induced higher mucosal immunity in *** absorption and phagocytosis of recombinant *** strains in IPEC-J2 cells or porcine alveolar macrophage(PAM)cells were significantly enhanced,***,we introduce a kind of cheap and safe oral ***-vectored vaccine,which could activate the specific mucosal immunity for controlling ASFV infection.