Genomic characterization of SARS-CoV-2 identified in a reemerging COVID-19 outbreak in Beijing's Xinfadi market in 2020

作     者：Yong Zhang Yang Pan Xiang Zhao Weifeng Shi Zhixiao Chen Sheng Zhang Peipei Liu Jinbo Xiao Wenjie Tan Dayan Wang William J.Liu Wenbo Xu Quanyi Wang Guizhen Wu Yong Zhang;Yang Pan;Xiang Zhao;Weifeng Shi;Zhixiao Chen;Sheng Zhang;Peipei Liu;Jinbo Xiao;Wenjie Tan;Dayan Wang;William J. Liu;Wenbo Xu;Quanyi Wang;Guizhen Wu

作者机构：NHC Key Laboratory of BiosafetyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Beijing Center for Disease Control and PreventionBeijing 100013China Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of ShandongShandong First Medical University and Shandong Academy of Medical SciencesTai'an 271000China The First Affiliated Hospital of Shandong First Medical University(Shandong Provincial Qianfoshan Hospital)Jinan 271099China 

出 版 物：《Biosafety and Health》 (生物安全与健康（英文）)

年 卷 期：2020年第2卷第4期

页      面：202-205页

学科分类：100208[医学-临床检验诊断学] 1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the National Science and Technology Major Project of China(Project No.2017ZX10104001 2018ZX10711001) 

主　　题：COVID-19 SARS-CoV-2 Genomic epidemiology L-lineage European branch I D614G mutation 

摘      要：After 56 days without coronavirus disease 2019(COVID-19)cases,reemergent cases were reported in Beijing,China on June 11,***,we report the genetic characteristics of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)sequenced from the clinical specimens of 4 human cases and 2 environmental *** nucleotide similarity among six SARS-CoV-2 genomes ranged from 99.98%to 99.99%.Compared with the reference strain of SARS-CoV-2(GenBank ***_045512),all six genome sequences shared the same substitutions at nt241(C→T),nt3037(C→T),nt14408(C→T),nt23403(A→G),nt28881(G→A),nt28882(G→A),and nt28883(G→C),which are the characteristic nucleotide substitutions of L-lineage European branch *** was also proved by themaximum likelihood phylogenetic tree based on the full-length genome of *** also have a unique shared nucleotide substitution,nt6026(C→T),which is the characteristic nucleotide substitution of SARS-CoV-2 in Beijing s Xinfadi *** is noteworthy that there is an amino acid D614Gmutation caused by nt23403 substitution in all six genomes,which may enhance the virus s infectivity in humans and help it become the leading strain of the virus to spread around the world *** is necessary to continuously monitor the genetic variation of SARS-CoV-2,focusing on the influence of key mutation sites of SARS-CoV-2 on viral transmission,clinical manifestations,severity,and course of disease.