Regulation of Rice Tillering by RNA-Directed DNA Methylation at Miniature Inverted-Repeat Transposable Elements
作者机构：Center for Plant BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China Tsinghua-Peking Center for Life SciencesBeijing 100084China Key Laboratory of Plant Genomics and National Center for Plant Gene ResearchInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Vector-borne Virus Research CenterState Key Laboratory of Ecological Pest Control for Fujian and Taiwan CropsFujian Province Key Laboratory of Plant VirologyInstitute of Plant VirologyFujian Agriculture and Forestry UniversityFuzhou 350002China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2020年第13卷第6期
页 面：851-863页
核心收录：
学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学]
基 金：This work was supported by grants from National Natural Science Foundation of China(grant no.31788103) to J.L.and Y.Q.and the National Key R&D Program of China(grant no.2016YFA0500800) to Y.Q.Y.Q.is a visiting investigator of the CAS Center for Excellence in Molecular Plant Sciences.
主 题：RdDM siRNA MITEs OsMIR156 D14 tiller
摘 要：Tillering is a major determinant of rice plant architecture and grain yield.Here,we report that depletion of rice OsNRPD1a and OsNRPD1b,two orthologs of the largest subunit of RNA polymerase IV,leads to a high-tillering phenotype,in addition to dwarfism and smaller panicles.OsNRPD1a and OsNRPD1b are required for the production of 24-nt small interfering RNAs that direct DNA methylation at transposable elements(TEs)including miniature inverted-repeat TEs(MITEs).Interestingly,many genes are regulated either positively or negatively by TE methylation.Among them,OsMIR156d and OsMIR156j,which promote rice tillering,are repressed by CHH methylation at two MITEs in the promoters.By contrast,D14,which suppresses rice tillering,is activated by CHH methylation at an MITE in its downstream.Our findings reveal regulation of rice tillering by RNA-directed DNA methylation at MITEs and provide potential targets for agronomic trait enhancement through epigenome editing.
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