Involvement of glycated albumin in adipose-derived-stem cellmediated interleukin 17 secreting T helper cell activation

作     者：Julien Pestel Maud Robert Sara Corbin Hubert Vidal Assia Eljaafari 

作者机构：INSERM U1060 CarMenBatiment CENS-ELICentre Hospitalier Lyon SudPierre Bénite 69310France Faculty of MedicineUniversitéClaude Bernard Lyon 1Batiment CENS-ELICentre Hospitalier Lyon SudPierre Bénite 69310France Department of Surgery in Gastro-enterologyEdouard Herriot HospitalLyon 69003France Public Health DepartmentHospices Civils de Lyon1 quai des célestins Lyon 69002France DO-IT Research TeamHospices Civils de Lyon1 quai des célestinsLyon 69002France 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2020年第12卷第7期

页      面：621-632页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Interleukin 17 secreting T helper cells Lean adipose tissue Type 1 diabetes Advanced glycation end products Adipose-derived stem cells 

摘      要：BACKGROUND Advanced glycation end products(AGE)are a marker of various diseases including diabetes,in which they participate to vascular damages such as retinopathy,nephropathy and *** those vascular complications,AGE are involved in altered metabolism in many tissues,including adipose tissue(AT)where they contribute to reduced glucose uptake and attenuation of insulin *** are known to contribute to type 1 diabetes(T1D)through promotion of interleukin(IL)-17 secreting T helper(Th17)*** To investigate whether lean adipose-derived stem cells(ASC)could be able to induce IL-17A secretion,with the help of *** As we have recently demonstrated that ASC are involved in Th17 cell promotion when they are harvested from obese AT,we used the same co-culture model to measure the impact of glycated human serum albumin(G-HSA)on human lean ASC interacting with blood mononuclear ***-17A and pro-inflammatory cytokine secretion were measured by *** of AGE(RAGE)together with intercellular adhesion molecule 1(ICAM-1),human leukocyte Antigen(HLA)-DR,cluster of differentiation(CD)41,and CD62P surface expressions were measured by ***-RAGE specific monoclonal antibody was added to co-cultures in order to evaluate the role of RAGE in IL-17A *** Results showed that whereas 1%G-HSA only weakly potentiated the production of IL-17A by T cells interacting with ASC harvested from obese subjects,it markedly increased IL-17A,but also interferon gamma and tumor necrosis factor alpha production in the presence of ASC harvested from lean *** was associated with increased expression of RAGE and HLA-DR molecule by cocultured ***,RAGE blockade experiments demonstrated RAGE specific involvement in lean ASC-mediated Th-17 cell ***,platelet aggregation and ICAM-1,which are known to be induced by AGE,were not involved in these *** Thus,our results demonstrated tha