Specific Patterns of Spinal Metabolite Ratio Underlying a-Me-5-HTevoked Pruritus Compared with Compound 48/80 Based on Proton Nuclear Magnetic Resonance Spectroscopy

作     者：Ying-le CHEN Zhi-gang HE Qian WANG Hong-bing XIANG Li FAN Jun XIONG Ying-le CHEN;Zhi-gang HE;Qian WANG;Hong-bing XIANG;Li FAN;Jun XIONG

作者机构：Department of Anesthesiologythe First Affiliated Quanzhou Hospital of Fujian Medical UniversityQuanzhou 362000China Emergency MedicineTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Anesthesiology and Pain MedicineTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of OrthopedicsUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Hepatobiliary Surgery CenterUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China 

出 版 物：《Current Medical Science》 (当代医学科学（英文）)

年 卷 期：2020年第40卷第4期

页      面：761-766页

核心收录：

学科分类：07[理学] 070202[理学-粒子物理与原子核物理] 0702[理学-物理学] 

基　　金：This work was supported by grants from the National Natural Science Foundation of China(No.81670240 and No.81873467) the Medical Innovation Project in Fujian Province(No.2017-CX-48) 

主　　题：itch pruritus intensity spinal cord metabolomics proton nuclear magnetic resonance 

摘      要：Summary:Mechanisms of pruritus are implicated in the dysregulation of the metabolites in the spinal *** investigated pruritus behavioral testing in three groups of young adult male C57B1/6 mice,including one group treated with normal saline,while the other groups intradermally injected with a-Me-5-HT(histamine-independent pruritogen),compound 48/80(histaminedependent pruritogen)at the nape skin of the neck,*** nuclear magnetic resonance spectroscopy(MRS)was used to compare spinal metabolites from the vertebral cervical among three groups,and to study the association of spinal metabolite ratio and pruritus *** MRS-measured N-acetylaspartate-to-myoinositol ratio(NAA/Ins)was significantly correlated with the number of scratches between normal saline group and 48/80 group or a-Me-5-HT group(both P0.0001),indicating that NAA/Ins may be a robust surrogate marker of histamine-independent/dependent *** was significant difference in Glu/Ins between normal saline group and 48/80 group(P=0.017),indicating that Glu/Ins may be a surrogate marker of histamine-dependent pruritogen,while GABA/Ins was highly significantly different between normal saline group and a-Me-5-HT group(P=0.008),suggesting that GABA/Ins may be a surrogate marker of histamineindependent *** may reflect the extent of pruritus intensity elicited by a-Me-5-HT and compound 48/80 with sensitivity similar to the number of scratches,and above potential markers need to be further validated in pre-clinical and clinical treatment trials.