Abscisic Acid Suppresses the Highly Occurred Somatic Homologous Recombination in Arabidopsis rfc1 Mutant

作     者：Tingxiu Yao Dan Jin Qian Liu Zhizhong Gong 

作者机构：State Key Laboratory of Plant Physiology and BiochemistryCollege of Biological SciencesChina Agricultural University National Center for Plant Gene Research 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2013年第40卷第9期

页      面：465-471页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：Abscisic acid DNA replication factor C1 Genomic stability 

摘      要：The phytohormone abscisic acid （ABA） regulates many aspects of division. Previous study indicates that ABA treatment increases DNA plant growth, including seed germination, root growth and cell damage and somatic homologous recombination （HR） in Arabi- dopsis abo4/pol ε （aba overly-sensitive 4/DNA polymerase ε） mutants. DNA replication factor C （RFC） complex is required for loading PCNA （Proliferating Cell Nuclear Antigen） during DNA replication. The defect in RFC1, the largest subunit of RFC, causes the high HR and DNA damage sensitivity in Arabidopsis. Here we found that like pol e/abo4, rfcl is sensitive to ABA in both ABA-inhibiting seed germination and root growth. However, ABA treatment greatly reduces HR and also reduces the expression of the DNA-damaged marker genes in ,rfcl. These results suggest that RFCI plays critical roles in ABA-mediated HR in Arabidopsis.