Emodin Inhibits Lipopolysaccharide-Induced Inflammation by Activating Autophagy in RAW 264.7 Cells

作     者：TU Yan-jie TAN Bo JIANG Lei WU Zhong-hua YU Hong-ji LI Xiao-qian YANG Ai-dong TU Yan-jie;TAN Bo;JIANG Lei;WU Zhong-hua;YU Hong-ji;LI Xiao-qian;YANG Ai-dong

作者机构：Research Centre on Application of Classical PrescriptionsBasic Medical CollegeShanghai University of Traditional Chinese MedicineShanghai 201203China Department of Febrile DiseaseBasic Medical CollegeShanghai University of Traditional Chinese MedicineShanghai 201203China Clinical Pharmacokinetic LaboratoryShuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 201203China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2021年第27卷第5期

页      面：345-352页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Ministry of Science and Technology of China(No.2018YFC1704102) the National Natural Science Foundation of China(Nos.81673855 and 81904072) 

主　　题：emodin lipopolysaccharide inflammation autophagy 

摘      要：Objective:To investigate the effects of emodin on inflammation and autophagy in lipopolysaccharide(LPS)-induced RAW 264.7 macrophages and reveal its underlying ***:3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2 H-tetrazolium(MTS)assay was conducted to find the appropriate dose for ***264.7 cells pretreated with different concentrations(0–50μmol/L)of emodin or vehicle for 2 h prior to exposure to LPS for 16 *** morphology was examined and propidium iodide staining was used to examine cell *** of inflammation-related proteins[nuclear factor-kappa B(NF-κB)and I-kappa B(IκB)α]and autophagy-related proteins[light chain(LC)3,P62/sequestosome 1,mammalian target of rapamycin(m TOR),and p-m TOR]were examined using Western blot *** of inflammation-related cytokines including tumor necrosis factor(TNF)-α,interleukin(IL)-1βand IL-6 were detected by enzyme-linked immunosorbent *** was examined with LC3 B fluorescence intensity and *** effect of emodin on autophagy was conducted with an autophagy inhibitor,3-methyladenine(3-MA).Results:The expression of NF-κB in LPS-induced cells was significantly increased(P0.01)and simultaneously IκBαdecreased compared with the normal cell(P0.05).The expressions of TNF-α,IL-1β,and IL-6 proteins in the LPS-induced RAW264.7 cells were significantly higher than in the normal cell(P0.05 or P0.01).LPS increased the percentage of cells in the G0/G1 phase,which was recovered by emodin at different doses(12.5,25,and 50μmol/L,P0.05 or P0.01).The mediumdose(25μml/L)emodin decreased the expressions of NF-κB,P62 and p-m TOR(P0.01)and increased IκBαexpression,LC3 BⅡ/Ⅰratio as well as LC3 B fluorescence intensity(P0.05 or P0.01).Meanwhile,the enhanced autophagic effects of emodin,such as the increment of LC3 BⅡ/ratio and the decrement of P62 expression,were suppressed by autophagy inhibitor ***:Emodin could inhibit inflammation of m