Y chromosome microdeletion screening using a new molecular diagnostic method in 1030 Japanese males with infertility
作者机构:Department of Integrative Cancer Therapy and UrologyKanazawa University Graduate School of Medical ScienceKanazawa 920-8641Japan Kyono ART ClinicSendai 980-0014Japan Kyono ART ClinicTakanawaTokyo 108-0074Japan Suzuki Lady's HospitalKanazawa 921-8033Japan Ebisu Tsuji ClinicTokyo 150-0021Japan Tenjin Tsuji ClinicFukuoka 810-0001Japan Dokkyo Medical University Koshigaya HospitalSaitama 343-8555Japan Department of UrologyFaculty of MedicineToho UniversityTokyo 143-8541Japan
出 版 物:《Asian Journal of Andrology》 (亚洲男性学杂志(英文版))
年 卷 期:2020年第22卷第4期
页 面:368-371页
核心收录:
主 题:azoospermia factor Japanese infertile men sperm recovery rate Y chromosome microdeletion
摘 要:The azoospermia factor(AZF)region is important for spermatogenesis,and deletions within these regions are a common cause of oligozoospermia and azoospermia.Although several studies have reported this cause,the present research,to the best of our knowledge,is the first large-scale study assessing this factor in Japan.In this study,1030 male patients with infertility who were examined for Y chromosome microdeletion using the polymerase chain reaction-reverse sequence-specific oligonucleotide(PCR-rSSO)method,a newly developed method for Y chromosome microdeletion screening,were included.The study enrolled 250 patients with severe oligospermia and 717 patients with azoospermia.Among the 1030 patients,4,4,10,and 52 had AZFa,AZFb,AZFb+c,and AZFc deletions,respectively.The sperm recovery rate(SRR)of microdissection testicular sperm extraction in patients with AZFc deletions was significantly higher than that in those without AZF deletions(60.0%vs 28.7%,P=0.04).In patients with gr/gr deletion,SRR was 18.7%,which was lower than that in those without gr/gr deletion,but was not statistically significant.In conclusion,our study showed that the frequency of Y chromosome microdeletion in male patients in Japan was similar to that reported in patients from other countries,and SRR was higher in patients with AZFc deletion.