L-asparaginase production by mangrove derived Bacillus cereus MAB5:optimization by response surface methodology

作     者：Thenmozhi C Sankar R Karuppiah V Sampathkumar P 

作者机构：Centre of Advanced Study in Marine BiologyFaculty of Marine Sciences Annamalai University Parangipettai-608 502Tamil NaduIndia 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2011年第4卷第6期

页      面：486-491页

核心收录：

学科分类：081703[工学-生物化工] 1002[医学-临床医学] 08[工学] 0817[工学-化学工程与技术] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

主　　题：L-asparaginase Bacillus cereus Mangroves Plackett-Barman design Central Composite design 

摘      要：Objective:To isolate marine bacteria,statistically optimize them for maximum asparaginase ***:In the present study,statistically based experimental designs were applied to maximize the production of L-asparaginase from bacterial strain of Bacillus cereus（***） MAB5（HQ675025） isolated and identified by 16S rDNA sequencing from mangroves rhizosphere ***:Plackett-Barman design was used to identify the interactive effect of the eight variables *** extract,soyabean meal,glucose,magnesium sulphate,KH2PO4,wood chips,aspargine and sodium *** the variables are denoted as numerical factors and investigated at two widely spaced intervals designated as -1（low level） and +1（high level）. The effect of individual parameters on L-asparaginase production was *** meal,aspargine,wood chips and sodium chloride were found to be the significant among eight *** maximum amount of L-asparaginase produced（51.54 IU/mL） from the optimized medium containing soyabean meal（6.282 8 g/L）,aspargine（5.5 g/L）,wood chips（1.383 8 g/L） and NaCl（4.535 4 g/L）.Conclusions:The study revealed that,it is useful to produce the maximum amount of L-asparaginase from *** MAB5 for the treatment of various infections and diseases.