The molecular interaction of ADAMTS-1 and fibulin-1 and its potential contribution to breast cancer biology

作     者：Yamina Mohamedi Tania Fontanil Teresa Cobo Jose A.Vega Juan L.Cobo Olivia García-Suárez Juan Cobo Santiago Cal Alvaro J.Obaya 

作者机构：Departamento de Bioquímica y Biología MolecularUniversidad de OviedoOviedo 33006AsturiasSpain Instituto Universitario de OncologíaIUOPAUniversidad de OviedoOviedo 33006AsturiasSpain Departamento de InvestigaciónClínica OrdonezOviedo 33012AsturiasSpain Instituto Asturiano de OdontologíaUniversidad de OviedoOviedo 33006AsturiasSpain Departamento de Cirugía y Especialidades Médico-QuirúrgicasUniversidad de OviedoOviedo 33006AsturiasSpain Departamento de Morfología y Biología CelularFacultad de MedicinaUniversidad de OviedoOviedo 33006AsturiasSpain Facultad de Ciencias de la SaludUniversidad Autónoma de ChileProvidenciaSantiago 7500912Chile Servicio de Cirugía MaxilofacialHospital Universitario Central de Asturias(HUCA)Oviedo 33011AsturiasSpain Departamento de Biología FuncionalArea de FisiologíaUniversidad de OviedoOviedo 33006AsturiasSpain 

出 版 物：《Journal of Cancer Metastasis and Treatment》 (癌症转移与治疗（英文版）)

年 卷 期：2019年第4卷第5期

页      面：1-15页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the Instituto Asturiano de Odontología(IAO).Mohamedi Y is recipient of a fellowship from the FICYT,"Fundación para el Fomento en Asturias de la Investigación Científica Aplicaday la Tecnología" Tania Fontanil is recipient of a contract from the"Departamento de Investigación de Clínica Ordonez(Oviedo)" 

主　　题：ADAMTS-1 fibulin-1 cell migration cell proliferation breast cancer MCF-7 MDA-MB-231 

摘      要：Aim: Fibulins and ADAMTSs are two families of extracellular matrix proteins implicated in key functional and pathological processes. The fact that the fibulin-1 and ADAMTS-1 proteins interact raises new questions about the roles of these extracellular matrix proteins in modulating tumor progression. Herein, we described the functional implications of the interaction between fibulin-1 and ADAMTS-1 on the behavior of breast cancer cell lines. Methods: Fibulin-1 and ADAMTS-1 were exogenously expressed in MCF-7 and MDA-MB-231 cell lines to assay the effect of their interaction in cellular properties. Results: ADAMTS-1 expression exacerbates tumor effects in terms of proliferation, invasion and mammosphere formation. In contrast, the simultaneous expression of ADAMTS-1 and fibulin-1 impairs these effects. The analysis of the expression of both proteins in human breast cancer tissue arrays provides new insights into the complex roles of fibulin-1 and ADAMTS-1 in this type of tumor. ;Conclusion: Our results suggests that the interaction between ADAMTS-1 and fibulin-1 induces a pronounced anti-tumoral effect.