Antidiabetic effect of Chrysophyllum albidum is mediated by enzyme inhibition and enhancement of glucose uptake via 3T3-L1 adipocytes and C2C12 myotubes
作者机构：Department of Pharmacognosy and Herbal MedicineSchool of PharmacyUniversity of Health and Allied SciencesHoGhana Department of PharmacognosyFaculty of Pharmacy and Pharmaceutical SciencesCollege of Health SciencesKwame Nkrumah University of Science and TechnologyKumasiGhana Department of Molecular MedicineSchool of Medical SciencesCollege of Health SciencesKwame Nkrumah University of Science and TechnologyKumasiGhana Department of Basic and Applied BiologySchool of SciencesUniversity of Energy and Natural ResourcesSunyaniGhana
出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)
年 卷 期：2020年第10卷第9期
页 面：387-396页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by some funds from KNUST Research Fund(KReF)
主 题：Antidiabetic C2C12 myotubes α-glucosidase inhibition α-amylase inhibition Glucose uptake Chrysophyllum albidum
摘 要：Objective:To investigate the in vivo and in vitro antidiabetic potential of Chrysophyllum ***:The effects of oral treatment with hydro-ethanolic extract(125,250 and 500 mg/kg)of the stem bark of Chrysophyllum albidum and glibenclamide for 21 d on glucose level,serum enzyme markers for liver function,lipid profile,total protein,serum urea,serum creatinine,and body weight were evaluated in experimental diabetic rats administered with 45 mg/kg of *** vitro assays including glucose uptake in C2 C12 cells and 3 T3-L1 adipose tissues,α-glucosidase andα-amylase inhibition were employed to evaluate the possible mechanism of hypoglycemic action of the *** and nitric oxide radical antioxidant activity of the extract was also ***:The increased levels of blood glucose,triglycerides,lowdensity lipoprotein,total cholesterol,serum aspartate,and alanine transaminases,creatinine,and urea in the diabetic animals were reduced significantly(P0.01)after treatment with Chrysophyllum albidum *** decreased total protein and high-density lipoprotein concentrations were normalized after *** addition,the extract significantly(P0.01)increased the transport of glucose in 3 T3-L1 cells and C2 C12 myotubes and exhibited considerable potential to inhibitα-amylase andα-*** also demonstrated potent antioxidant action by scavenging considerably DPPH and nitric oxide ***:Chrysophyllum albidum stem bark extract exhibits considerable antidiabetic effect by stimulating glucose uptake and utilization in C2 C12 myotubes and 3 T3-L1 adipocytes as well as inhibiting the activities ofα-amylase andα-glucosidase.
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