Effect of Tongxinluo on basic fibroblast growth factor mRNA after cerebral ischemia/reperfusion injury

作     者：Ruixue Yin Bingxun Lu Lixin Wang Jianzhong Fan Changjun Lu Hongzhen Zhou 

作者机构：Department of Neurology Nanfang Hospital of Southem Medical University Guangzhou 510515 Guangdong Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2006年第1卷第6期

页      面：509-512页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：the National Nat-ural Science Foundation of Chi-na  No. 30400612 

主　　题：cerebral ischemia time point polymerase chain reaction fibroblast growth factor middle cerebral artery reverse transcription analysis of variance Experimental Animal neural stem cells distilled water Wistar rats basic value 

摘      要：BACKGROUND ： Basic fibroblast growth factor （bFGF） can cause proliferation and differentiation of plentiful cells which are derived from mesoblast and neural ectoblast. Previous researches suggested that Tongxinluo could promote differentiation from neural stem cells （NSCs） to neurons and astrocytes after cerebral ischemia/reperfusion injury. OBJECTIVE： To observe the effect of Tongxinluo on bFGF mRNA of rats after cerebral ischemia/reperfusion injury and analyze its dosage-dependence. DESIGN ： Randomized controlled study SETTING ： Department of Neurology, Nanfang Hospital of Southem Medical University MATERIALS： The experiment was carried out in Neurological Department of Nanfang Hospital affiliated to Southern Medical University from May 2005 to March 2006. A total of 192 healthy Wistar rats, weighing （200±20） g, of SPF grade, of both gender by half, were provided by Experimental Animal Center of Nanfang Hospital Affiliated to Southern Medical University. Tongxinluo was consisted of renshen, wugong, quanxie, shuizhi, chishao, bingpian, etc. and was provided by Shijiazhuang Yiling Pharmaceutical Co., Ltd. （batch number： 9906115）. Main reagents： Agarose, DEPC-treated water, total RNA purification kit and one-step reverse transcription polymerase chain reaction （RT-PCR） kit were provided by Beijing Saibaisheng Gene Technique Co., Ltd. METHODS ： All rats were randomly divided into 4 groups, including high-dosage Tongxinluo group, low-dosage Tongxinluo group, model group and sham operation group with 48 in each group. Rats in the former three groups were observed at 3, 5, 7, 14, 21 and 30 days after ischemia/reperfusion. In addition, rats in sham operation group were selected at the relevant time points. Every 8 rats were selected from each group for once. （1） Right middle cerebral artery occlusion-reperfusion models were induced by Koizumi method and modified; moreover, neurological impairment was evaluated with Zealonga method. Except rats in sham operation