Transcriptomics of Chinese Sapium Sebiferum(L.)Roxb seed to reveal key enzymes involved in oil accumulation

作     者：Yanyan Yu Yun Liu Yanyan Yu;Yun Liu

作者机构：College of Life Science and TechnologyBeijing University of Chemical TechnologyBeijingChina Beijing Zest Bridge Media Technology Inc.BeijingChina 

出 版 物：《Oil Crop Science》 (中国油料作物学报(英文版）)

年 卷 期：2020年第5卷第3期

页      面：107-113页

学科分类：09[农学] 0901[农学-作物学] 

基　　金：funded by the National Science&Technology Pillar Program during the Twelfth Five-year Plan Period of China(2011BAD22B08) 

主　　题：Sapium sebiferum(L.)Roxb Transcriptome Differentially expressed analysis Lipid metabolism 

摘      要：Chinese Sapium sebiferum(L.)Roxb has great potential to emerge as a promising biodiesel resource plant due to abundant oil content in ***,the unavailability of transcriptomic and genomic data impedes the progress of application in biodiesel *** analysis will help to further exploit tallow and kernel oil utilization in industry and facilitate molecular-breeding-based genetic improvement of *** hiseq 2000 sequencing platform was used to perform de novo transcriptome assembly and gene expression analyses of seeds from three strains in three developmental stages.A total of 188,254 unigenes over 200 bp were assembled with average length of 699 *** transcriptic unigenes were functionally assigned by GO and KEGG *** was used to identify differentially expressed unigenes of five cDNA *** genes endcoding enzymes related to fatty acid metabolism were ***-PCR was employed to confirm five key enzymes involved in fatty acid and triacylglycerol *** study provided comprehensive gene expression information of Chinese *** seed at transcriptional *** transcriptome data will facilitate high-oil-content genetic cultivation of ***,which will further exploit tallow and kernel oil utilization in biodiesel industry.