Assessing combined toxic effects of tetracycline and P25 titanium dioxide nanoparticles using Allium cepa bioassay

作     者：Barsha Roy Khushboo Kadam Suresh Palamadai Krishnan Chandrasekaran Natarajan Amitava Mukherjee Barsha Roy;Khushboo Kadam;Suresh Palamadai Krishnan;Chandrasekaran Natarajan;Amitava Mukherjee

作者机构：School of Biosciences and TechnologyVITVellore 632014India Centre for NanobiotechnologyVITVellore 632014India 

出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)

年 卷 期：2021年第15卷第1期

页      面：101-112页

核心收录：

学科分类：07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：We would like to acknowledge VIT for providing research facilities. There are no conflicts of interest to declare 

主　　题：Binary toxicity Cell viability Mitotic index Chromosomal aberration Reactive oxygen species Nanoparticles internalization 

摘      要：Usage of titanium dioxide nanoparticles(TiO_(2) NPs)and tetracycline(TC)has increased significantly in the present *** leads to their release and accumulation in the *** the compounds,individually,can have adverse toxic effects on the *** binary mixtures can increase this degree of *** present study aimed to evaluate the toxicity of both the contaminants in individual and binary mixtures in Allium ***,the toxicity of TiO_(2) NPs upon UVA pre-irradiation was also *** showed that UVA pre-irradiated NPs(UVA-TiO_(2) NPs)had a significant decrease in cell viability than their non-irradiated counterparts(NI-TiO_(2)),denoting an increase in photocatalytic activity upon UVA *** low concentrations of TC(EC10=0.016µmol/L)mixed with TiO2 NPs significantly increased the toxicity for both UVA-TiO_(2) and NI-TiO_(2) *** ROS generation was significantly high for UVA-TiO_(2) ***,TC did not have any effects on ROS *** the compounds exhibited genotoxic potential in *** chromosomal abnormalities like anaphase bridges,telophase bridges,laggard chromosomes,binucleate cells,*** *** binary mixture of UVA-TiO_(2) NPs and TC showed the highest chromosomal aberrations(64.0%±1.26%)than the mixture with NI-TiO_(2) or the individual *** decreased significantly after recovery(46.8%±1.92%),denoting the self-repair *** study proved that UVA-TiO2 NPs were more toxic and could be enhanced further when mixed with a sub-lethal concentration of *** work will help to assess the risk of both compounds in the environment.