Rapid construction of a whole-genome mutant library by combining haploid stem cells and inducible self-inactivating PiggyBac transposon

作     者：Junjie Mao Kai Xu Jiabao Han Guihai Feng Ying Zhang Wei Li Junjie Mao;Kai Xu;Jiabao Han;Guihai Feng;Ying Zhang;Wei Li

作者机构：State Key Laboratory of Stem Cell and Reproductive BiologyInstitute of ZoologyChinese Academy of SciencesBeijing 100101China Institute for Stem Cell and RegenerationChinese Academy of SciencesBeijing 100101China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2020年第11卷第6期

页      面：452-457页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：supported by the National Key Research and Development Program the Strategic Priority Research Program of the Chinese Academy of Sciences 国家自然科学基金 the Key Research Projects of the Frontier Science of the Chinese Academy of Sciences 

主　　题：PiggyBac inducible inactivating 

摘      要：Dear Editor,Genome-scale screening is a powerful method used to explore phenotypes that are of interest,and numerous screening systems have been built for functional sites identification.Classical genetic screening in eukaryotes has been performed using chemical mutagens(Chen et al.,2000),transposon mediated gene trapping(Dupuy et al.,2005),and CRISPR-Cas9 system(Wang et al.,2014).