Dual roles of juvenile hormone signaling during early oogenesis in Drosophila

作     者：Wei Luo Sethuraman Veeran Jian Wang Sheng Li Kang Li Su-Ning Liu Wei Luo;Sethuraman Veeran;Jian Wang;Sheng Li;Kang Li;Su-Ning Liu

作者机构：Guangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and Technology&School of Life SciencesSouth China Normal UniversityGuangzhouChina and 2 Department of EntomologyUniversity of MarylandCollege ParkMDUSA 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2020年第27卷第4期

页      面：665-674页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：We thank Dr.Jian-Hua Huang for flies and helpful comments on this manuscript.This work was supported by the National Natural Science Foundation of China(Grant No.31702054). 

主　　题：cap cells germline stem cells juvenile hormone oogenesis ovary 

摘      要：Juvenile hormone(JH)signaling plays crucial roles in insect metamorphosis and reproduction.Function of JH signaling in germline stem cells(GSCs)remains largely unknown.Here,we found that the number of GSCs significantly declined in the ovaries of Met,Gee and JHAMT mutants.Then we inhibited JH signaling in selected cell types of ovaries by expressing Met and Gee or Kr-hl double-stranded RNAs(dsRNAs)using different Gal4 drivers.Blocking of JH signaling in muscle cells has no effect on GSC numbers.Blocking of JH signaling in cap cells reduced GSCs cells.Inductive expression of Met and Gee dsRNA but not Kr-hl by Nos-Gal4 increased GSC cells.These results indicate that JH signaling plays an important role in GSC maintenance.